Bonney R J, Hopkins H A, Walker P R, Potter V R
Biochem J. 1973 Sep;136(1):115-24. doi: 10.1042/bj1360115.
Intact rats trained on a controlled feeding and lighting schedule designated ;8+16' exhibited diurnal oscillations in liver weight, glucokinase activity and liver glycogen content. Glucokinase activity expressed as units/g of liver decreased to 30% of that from unoperated controls during the first 48h after partial hepatectomy and returned to near normal values in 2 weeks. When the glucokinase activity was expressed as units/liver per 100g body wt., a decrease to 50% of control activity was observed between 24 and 48h after the operation. A similar pattern was found for pyruvate kinase type I. In contrast, pyruvate kinase type III activity increased after partial hepatectomy. It is suggested that the newly divided cells after partial hepatectomy do not synthesize glucokinase and pyruvate kinase I but do synthesize pyruvate kinase III. Glycogen was found to accumulate as early as 24h after partial hepatectomy, and normal concentrations were reached after 48h if the operation was performed at times other than during the feeding periods.
在指定为“8 + 16”的受控喂食和光照时间表下训练的完整大鼠，其肝脏重量、葡萄糖激酶活性和肝脏糖原含量呈现昼夜振荡。部分肝切除术后的头48小时内，以每克肝脏单位数表示的葡萄糖激酶活性降至未手术对照组的30%，并在2周内恢复至接近正常值。当葡萄糖激酶活性以每100克体重每肝脏单位数表示时，术后24至48小时观察到活性降至对照活性的50%。丙酮酸激酶I型也发现了类似的模式。相比之下，部分肝切除术后丙酮酸激酶III型活性增加。这表明部分肝切除术后新分裂的细胞不合成葡萄糖激酶和丙酮酸激酶I，但会合成丙酮酸激酶III。早在部分肝切除术后24小时就发现糖原开始积累，如果手术在非喂食期进行，48小时后可达到正常浓度。
