Lesslie I W, Herbert C N
Vet Rec. 1975 Apr 12;96(15):338-41. doi: 10.1136/vr.96.15.338.
A field trial on a country-wide basis was undertaken to compare the specificity for bovine tuberculosis of single and comparative tuberculin tests in cattle using either Weybridge human or Weybridge bovine PPD. The tests were made on 10,305 cattle in 179 herds distributed throughout all regions of England, Scotland and Wales. Results showed that a comparative tuberculin test using avian PPD with either human or bovine PPD had a much higher efficiency than a single injection of mammalian tuberculin in the neck of cattle, and confirmed that a comparative test is still essential in the British environment. Weybridge bovine PPD gave significantly better discrimination between tuberculous and non-tuberculous cattle than Weybridge human PPD when used together with avian PPD in a comparative tuberculin test. The diameter of induration gave an absolute measure of the extent of oedema, if present, and induration diameter used in conjunction with skin thickening increased the sensitivity and specificity of the test. Rules of interpretation were developed and are presented for an intradermal comparative tuberculin test in cattle using Weybridge avian and bovine PPDs.
在全国范围内进行了一项田间试验，以比较使用韦布里奇人用或韦布里奇牛用结核菌素纯蛋白衍生物（PPD）对牛进行单次和比较结核菌素试验时对牛结核病的特异性。对分布在英格兰、苏格兰和威尔士所有地区的179个牛群中的10305头牛进行了检测。结果表明，在牛颈部使用禽PPD与人类或牛PPD进行比较结核菌素试验，其效率远高于单次注射哺乳动物结核菌素，并证实了在英国环境下比较试验仍然至关重要。在比较结核菌素试验中，当与禽PPD一起使用时，韦布里奇牛用PPD在区分患结核病和未患结核病的牛方面比韦布里奇人用PPD具有明显更好的辨别能力。硬结直径给出了水肿程度（若存在）的绝对测量值，硬结直径与皮肤增厚一起使用可提高试验的敏感性和特异性。制定并给出了使用韦布里奇禽用和牛用PPD对牛进行皮内比较结核菌素试验的解读规则。
