Awah-Ndukum J, Kudi A C, Bah G S, Bradley G, Tebug S F, Dickmu P L, Njakoi H N, Agharih W N
School of Veterinary Medicine and Sciences, University of Ngaoundere, Ngaoundere, Adamawa Region, Cameroon.
Vet Med Int. 2012;2012:798502. doi: 10.1155/2012/798502. Epub 2012 Apr 1.
The aim of this study was to obtain epidemiological estimates of bovine tuberculosis (TB) prevalence in cattle in the highlands of Cameroon using two population-based tuberculin skin test (TST) surveys in the years 2009 and 2010. However, prior to the TST survey in 2010, blood was collected from already chosen cattle for serological assay. Anti-bovine TB antibodies was detected in 37.17% of tested animals and bovine TB prevalence estimates were 3.59%-7.48%, 8.92%-13.25%, 11.77%-17.26% and 13.14%-18.35% for comparative TST at ≥4 mm, ≥3 mm and ≥2 mm cut-off points and single TST, respectively. The agreement between TST and lateral flow was generally higher in TST positive than in TST negative subjects. The K coefficients were 0.119, 0.234, 0.251 and 0.254 for comparative TST at ≥4 mm, ≥3 mm and ≥2 mm cut-off points and the single TST groups, respectively. Chi square statistics revealed that strong (P < 0.05; χ(2) > 48) associations existed between seroprevalence rates and TST reactors. The study suggested that using lateral flow assay and TST at severe interpretations could improve the perception of bovine TB in Cameroon. The importance of defining TST at modified cut-offs and disease status by post-mortem detection and mycobacterial culture of TB lesions in local environments cannot be overemphasised.
本研究的目的是通过2009年和2010年两次基于人群的结核菌素皮肤试验（TST）调查，获得喀麦隆高地牛群中牛结核病（TB）患病率的流行病学估计值。然而，在2010年TST调查之前，已从选定的牛身上采集血液进行血清学检测。在37.17%的受试动物中检测到抗牛结核抗体，对于比较TST，在≥4mm、≥3mm和≥2mm的截断点以及单次TST时，牛结核病患病率估计值分别为3.59%-7.48%、8.92%-13.25%、11.77%-17.26%和13.14%-18.35%。TST阳性受试者中TST与侧向流动试验之间的一致性通常高于TST阴性受试者。对于比较TST，在≥4mm、≥3mm和≥2mm截断点以及单次TST组，K系数分别为0.119、0.234、0.251和0.254。卡方统计显示，血清阳性率与TST反应者之间存在强关联（P<0.05；χ(2)>48）。该研究表明，在严格的解释下使用侧向流动试验和TST可以提高喀麦隆对牛结核病的认知。通过在当地环境中对结核病变进行尸检检测和分枝杆菌培养来确定修改后的截断点的TST和疾病状态的重要性再怎么强调也不为过。
