核糖核酸酶A与表面活性剂之间的相互作用。
The interaction between ribonuclease A and surfactants.
作者信息
Jones M N, Skinner H A, Tipping E, Wilkinson A
出版信息
Biochem J. 1973 Sep;135(1):231-6. doi: 10.1042/bj1350231.
Abstract
- U.v. difference spectra show that the anionic surfactant sodium n-dodecyl sulphate unfolds ribonuclease A at pH7.3 and 10.3, but that the cationic surfactant n-dodecyltrimethylammonium bromide does not affect the conformation of the enzyme. 2. Equilibrium-dialysis experiments show that sodium n-dodecyl sulphate binds to ribonuclease A, but no binding of n-dodecyltrimethylammonium bromide could be detected at pH7.3. 3. The enzymic activity of ribonuclease A is unaffected by n-dodecyltrimethylammonium bromide up to a concentration of 0.03m at 25 degrees C. 4. Ultracentrifuge studies support the conclusion that n-dodecyltrimethylammonium bromide does not interact significantly with ribonuclease A. 5. The enthalpy change as measured by microcalorimetry on binding of sodium n-dodecyl sulphate to ribonuclease A is consistent with an exothermic enthalpy of binding occurring simultaneously with an endothermic enthalpy of chain unfolding.
摘要
- 紫外差光谱表明，阴离子表面活性剂正十二烷基硫酸钠在pH7.3和10.3时会使核糖核酸酶A展开，但阳离子表面活性剂正十二烷基三甲基溴化铵不会影响该酶的构象。2. 平衡透析实验表明，正十二烷基硫酸钠与核糖核酸酶A结合，但在pH7.3时未检测到正十二烷基三甲基溴化铵的结合。3. 在25℃下，浓度高达0.03m时，正十二烷基三甲基溴化铵对核糖核酸酶A的酶活性没有影响。4. 超速离心研究支持了正十二烷基三甲基溴化铵与核糖核酸酶A没有显著相互作用的结论。5. 通过微量量热法测量，正十二烷基硫酸钠与核糖核酸酶A结合时的焓变与结合的放热焓变同时发生链展开的吸热焓变一致。
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