Bacopoulos N G, Hattox S E, Roth R H
Eur J Pharmacol. 1979 Jun 15;56(3):225-36. doi: 10.1016/0014-2999(79)90175-4.
The concentrations of dopamine (DA) metabolites (free and conjugated) was measured in plasma and brain regions of rats by the mass spectrometric method of selected ion monitoring. Experimental treatments which altered the function of central dopamine neurons also induced concomitant changes in plasma 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA). Stimulation of the nigrostriatal pathway increased plasma DOPAC and HVA whereas lesion of the pathway decreased plasma metabolites. Several drug treatments induced parallel changes in brain and plasma concentrations of DA metabolites. It is suggested that changes in the concentration of DOPAC and HVA in rat brain are reflected by parallel changes in plasma. No conjugated forms of DOPAC and HVA were found in plasma and brain tissue of vervet monkeys (Cercopithecus aethiops).
采用选择离子监测质谱法测定大鼠血浆和脑区中多巴胺（DA）代谢物（游离型和结合型）的浓度。改变中枢多巴胺能神经元功能的实验处理也会引起血浆3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）的相应变化。黑质纹状体通路受到刺激会使血浆DOPAC和HVA升高，而该通路受损则会使血浆代谢物水平降低。几种药物处理会使脑和血浆中DA代谢物浓度发生平行变化。这表明大鼠脑中DOPAC和HVA浓度的变化会在血浆中得到相应反映。在黑长尾猴（非洲绿猴）的血浆和脑组织中未发现DOPAC和HVA的结合形式。
