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异硫氰酸酯对3-磷酸甘油醛脱氢酶必需巯基的选择性可逆修饰。

Selective and reversible modification of essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase by isothiocyanates.

作者信息

Gemeiner P, Drobnica L

出版信息

Experientia. 1979 Jul 15;35(7):857-9. doi: 10.1007/BF01955110.

DOI:10.1007/BF01955110
PMID:477830
Abstract

Aralkyl isothiocyanates, like aryl isothiocyanates, undergo a selective and reversible reaction with essential thiol groups of D-glyceraldehyde-3-phosphate dehydrogenase; the former 2 substances require for a reversible reaction course of more alkaline medium and presence of a thiol.

摘要

芳烷基异硫氰酸酯与芳基异硫氰酸酯一样，可与3-磷酸甘油醛脱氢酶的必需巯基发生选择性可逆反应；前两种物质的可逆反应过程需要更碱性的介质和巯基的存在。

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本文引用的文献

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THE ACYL-ENZYME INTERMEDIATE AND THE KINETIC MECHANISM OF THE GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE REACTION.甘油醛-3-磷酸脱氢酶反应的酰基-酶中间体及动力学机制
J Biol Chem. 1965 Feb;240:844-55.
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Monodispersity and quaternary structure of glyceraldehyde 3-phosphate dehydrogenase.
Biochemistry. 1968 Mar;7(3):919-26. doi: 10.1021/bi00843a006.
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Ligand induced half-of-the-sites reactivity in rabbit muscle glyceraldehyde-3-phosphate dehydrogenase.配体诱导兔肌甘油醛-3-磷酸脱氢酶的半位点反应性。
亲电物质对（钠/钾）-ATP酶的抑制作用：功能意义
Mol Cell Biochem. 1995;147(1-2):187-92. doi: 10.1007/BF00944800.
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Half-of-the-sites and all-of-the-sites reactivity in rabbit muscle glyceraldehyde 3-phosphate dehydrogenase.
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Mechanism of alkylation of rabbit muscle glyceraldehyde 3-phosphate dehydrogenase.兔肌肉磷酸甘油醛脱氢酶的烷基化机制
Biochemistry. 1971 Jun 22;10(13):2456-66. doi: 10.1021/bi00789a005.
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Simple alkanethiol groups for temporary blocking of sulfhydryl groups of enzymes.
Biochemistry. 1975 Feb 25;14(4):766-71. doi: 10.1021/bi00675a019.
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Reactions of cellulose isothiocyanates with thiol and amino compounds.异硫氰酸纤维素酯与硫醇和氨基化合物的反应。
Carbohydr Res. 1977 Feb;53(2):217-22. doi: 10.1016/s0008-6215(00)88089-1.