Gemeiner P, Augustín J, Drobnica L
Carbohydr Res. 1977 Feb;53(2):217-22. doi: 10.1016/s0008-6215(00)88089-1.
A cellulose isothiocyanate has been prepared by treatment of cellulose with 2,4-di-isocyanatotoluene followed by hydrolysis and reaction of the resulting amine with thiophosgene. The cellulose isothiocyanate was characterized by its binding capacity with respect to [14C]-glycine, [131 I]-human serum albumin, and 2-mercaptoethanol. An analytical method for binding capacity, based on reaction with [35 S]-alpha-toluenethiol, was developed. Because of the aromatic character of the NCS group of the cellulose isothiocyanate, the covalently bonded thiol can be quantitatively liberated.
通过用2,4 - 二异氰酸甲苯酯处理纤维素，随后进行水解，并使所得胺与硫光气反应，制备了一种纤维素异硫氰酸酯。该纤维素异硫氰酸酯通过其与[14C] - 甘氨酸、[131I] - 人血清白蛋白和2 - 巯基乙醇的结合能力来表征。基于与[35S] - α - 甲苯硫醇反应开发了一种结合能力的分析方法。由于纤维素异硫氰酸酯的NCS基团的芳香特性，共价结合的硫醇可以被定量释放。
