Reyes A, Oliphant G, Brackett B G
Fertil Steril. 1975 Feb;26(2):148-57.
Efforts have been directed toward partial purification and identification of a reversible decapacitation factor from rabbit seminal plasma. Five fractions were consistently obtained following fractionation of rabbit seminal plasma on a Sephadex G-200 column. Only the first two fractions had decapacitation factor (DF) activity. On SDS polyacrylamide gel electrophoresis, several carbohydrate-and protein-containing components were found in each fraction from the Sephadex column. Further, DF activity could be obtained in the precipitate resulting from cetyl pyridinium chloride (CPC) treatment of the active fractions from the Sephadex G-200 column. In the comparison of the components observed with SDS gel electrophoresis throughout these fractionation procedures, the reversible decapacitation activity appeared to be associated with a component containing protein and carbohydrate. From these studies, then, reversible decapacitation activity of rabbit seminal plasma appears to be associated with a glycoprotein of an approximate molecular weight of 115,000.
研究人员致力于从兔精浆中对一种可逆去能因子进行部分纯化和鉴定。兔精浆在葡聚糖凝胶G - 200柱上进行分级分离后，始终能得到五个级分。只有前两个级分具有去能因子（DF）活性。在SDS聚丙烯酰胺凝胶电泳中，从葡聚糖凝胶柱得到的每个级分中都发现了几种含碳水化合物和蛋白质的成分。此外，用十六烷基氯化吡啶（CPC）处理葡聚糖凝胶G - 200柱上的活性级分所得到的沉淀物中可获得DF活性。在整个这些分级分离过程中，通过SDS凝胶电泳观察到的成分比较中，可逆去能活性似乎与一种含蛋白质和碳水化合物的成分有关。因此，从这些研究来看，兔精浆的可逆去能活性似乎与一种分子量约为115,000的糖蛋白有关。
