λ噬菌体imm434 DNA的EcoRI片段的一级结构，包含噬菌体434的cI - cro区域和噬菌体λ的cII - o区域。

Primary structure of an EcoRI fragment of lambda imm434 DNA containing regions cI-cro of phage 434 and cII-o of phage lambda.

作者信息

Ovchinnikov Y A, Guryev S O, Krayev A S, Monastyrskaya G S, Skryabin K G, Sverdlov E D, Zakharyev V M, Bayev A A

出版信息

Gene. 1979 Jul;6(3):235-49. doi: 10.1016/0378-1119(79)90060-x.

Abstract

Digestion of phage lambda imm434 DNA with restriction endonuclease EcoRI yields 7 fragments. The shortest among them (1287 bp) contains the right part of the phage 434 immunity region and the phage DNA portion proximal to it. The complete primary structure of this fragment has been determined using the chemical method of DNA sequencing. Hypothetical amino-acid sequences of proteins coded by the cro gene of phage 434 and the cII gene of phage lambda, as well as NH2-terminal amino-acid sequences of the cI protein of phage 434 and the O protein of phage lambda, have been deduced solely on the basis of the DNA sequence. The fragment studied contains also the pR and probably prm promoters and the oR operator of phage 434. The sequence coding for them differs from the respective DNA sequence of phage lambda.

摘要

用限制性内切酶EcoRI消化噬菌体λ imm434 DNA可产生7个片段。其中最短的片段（1287碱基对）包含噬菌体434免疫区域的右侧部分及其近端的噬菌体DNA部分。已使用DNA测序化学方法确定了该片段的完整一级结构。仅根据DNA序列推导了噬菌体434的cro基因和噬菌体λ的cII基因编码的蛋白质的假设氨基酸序列，以及噬菌体434的cI蛋白和噬菌体λ的O蛋白的NH2末端氨基酸序列。所研究的片段还包含噬菌体434的pR和可能的prm启动子以及oR操纵子。编码它们的序列与噬菌体λ的相应DNA序列不同。