大肠杆菌RNA聚合酶的一级结构、rpoC基因的核苷酸序列及β'亚基的氨基酸序列。
The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit.
作者信息
Monastyrskaya G S, Gubanov V V, Guryev S O, Salomatina I S, Shuvaeva T M, Lipkin V M, Sverdlov E D
出版信息
Nucleic Acids Res. 1982 Jul 10;10(13):4035-44. doi: 10.1093/nar/10.13.4035.
Abstract
The primary structure of the E. coli rpoC gene (5321 base pairs) coding the beta'-subunit of RNA polymerase as well as its adjacent segment have been determined. The structure analysis of the peptides obtained by cleavage of the protein with cyanogen bromide and trypsin has confirmed the amino acid sequence of the beta'-subunit deduced from the nucleotide sequence analysis. The beta'-subunit of E. coli RNA polymerase contains 1407 amino acid residues. Its translation is initiated by codon GUG and terminated by codon TAA. It has been detected that the sequence following the terminating codon is strikingly homologous to known sequences of rho-independent terminators.
摘要
编码RNA聚合酶β'亚基的大肠杆菌rpoC基因（5321个碱基对）的一级结构及其相邻片段已被确定。用溴化氰和胰蛋白酶裂解该蛋白质所获得的肽段的结构分析，证实了从核苷酸序列分析推导出来的β'亚基的氨基酸序列。大肠杆菌RNA聚合酶的β'亚基含有1407个氨基酸残基。其翻译由密码子GUG起始，由密码子TAA终止。已检测到终止密码子之后的序列与已知的不依赖ρ因子的终止子序列显著同源。
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