Davey G, Smith J M, Kalmakoff J
Infect Immun. 1973 Dec;8(6):882-6. doi: 10.1128/iai.8.6.882-886.1973.
A substance toxic for mice has been extracted from the washed mycelia of Mortierella wolfii. This toxin could also be demonstrated in culture fluids after spontaneous autolysis of the fungal hyphae. Purification of the toxin was carried out using Sephadex chromatography (G-200, G-50) and resulted in a single band on sodium dodecyl sulfate polyacrylamide gels. Properties of the toxin included heat lability at 60 C, trypsin sensitivity, activity over a wide pH range, and a molecular weight of 23,600. The mean lethal dose for 16-week-old male mice was 3.3 mug of protein.
已从沃尔夫被孢霉洗涤后的菌丝体中提取出一种对小鼠有毒的物质。在真菌菌丝体自然自溶后的培养液中也能检测到这种毒素。使用葡聚糖凝胶色谱法（G - 200、G - 50）对毒素进行纯化，在十二烷基硫酸钠聚丙烯酰胺凝胶上得到一条单一谱带。该毒素的特性包括在60℃时热不稳定、对胰蛋白酶敏感、在较宽的pH范围内有活性，分子量为23,600。16周龄雄性小鼠的平均致死剂量为3.3微克蛋白质。
