Tas J
Histochem J. 1975 Jan;7(1):1-19. doi: 10.1007/BF01004829.
The influence of different histochemical conditions on some metachromatic staining reactions has been studied using polyacrylamide films containing pure glycostaminoglycans. The films were incubated in fixatives without staining, and in glycerol, diethylene glycol and other glycols, formamide, N,N-dimethylformamide, dimethyl sulphoxide and ethanol (of several concentrations) after staining and their absorption (metachromatic) spectra recorded. In the case of heparin and heparan sulphate the metachromasy was disturbed when the films were immersed before staining in some fixative solutions containing formaldehyde and acid. After equilibration of stained films in organic solvents, changes in the absorption peaks were found to depend on the type and concentration of solvent, the type of glycosaminoglycan and the type of dye. Films containing glycosaminoglycan plus protein were used to investigate the blocking of the metachromatic reaction as the result of ionic interactions with proteins. The parameters that influence this phenomenon (e.g type of protein, glycosaminoglycan and dye, pH of staining) are discussed and a three-dimensional picture is introduced which can explain some of the results obtained in these experiments.
利用含有纯糖胺聚糖的聚丙烯酰胺薄膜，研究了不同组织化学条件对某些异染性染色反应的影响。将薄膜在无染色的固定剂中孵育，并在染色后于甘油、二甘醇及其他二醇、甲酰胺、N，N -二甲基甲酰胺、二甲基亚砜和几种浓度的乙醇中孵育，记录其吸收（异染性）光谱。对于肝素和硫酸乙酰肝素，当薄膜在染色前浸入某些含甲醛和酸的固定剂溶液中时，异染性会受到干扰。在有机溶剂中平衡染色后的薄膜后，发现吸收峰的变化取决于溶剂的类型和浓度、糖胺聚糖的类型以及染料的类型。含有糖胺聚糖加蛋白质的薄膜用于研究与蛋白质发生离子相互作用导致异染性反应受阻的情况。讨论了影响该现象的参数（如蛋白质、糖胺聚糖和染料的类型、染色的pH值），并引入了一个三维图，该图可以解释这些实验中获得的一些结果。
