多种放线菌的rpsL和rsmG突变体产生抗生素过量
Antibiotic overproduction by rpsL and rsmG mutants of various actinomycetes.
作者信息
Tanaka Yukinori, Komatsu Mamoru, Okamoto Susumu, Tokuyama Shinji, Kaji Akira, Ikeda Haruo, Ochi Kozo
机构信息
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
出版信息
Appl Environ Microbiol. 2009 Jul;75(14):4919-22. doi: 10.1128/AEM.00681-09. Epub 2009 May 15.
Abstract
Certain streptomycin resistance mutations (i.e., rpsL and rsmG) result in the overproduction of antibiotics in various actinomycetes. Moreover, rpsL rsmG double-mutant strains show a further increase in antibiotic production. rpsL but not rsmG mutations result in a marked enhancement of oligomycin production in Streptomyces avermitilis and erythromycin production in Saccharopolyspora erythraea, accompanied by increased transcription of a key developmental regulator gene, bldD, in the latter organism.
摘要
某些链霉素抗性突变（即rpsL和rsmG）会导致各种放线菌中抗生素的过量产生。此外，rpsL rsmG双突变菌株显示抗生素产量进一步增加。rpsL突变而非rsmG突变导致阿维链霉菌中寡霉素产量显著提高，以及糖多孢红霉菌中红霉素产量显著提高，同时在后者中一个关键发育调节基因bldD的转录增加。
相似文献
1
Antibiotic overproduction by rpsL and rsmG mutants of various actinomycetes.
Appl Environ Microbiol. 2009 Jul;75(14):4919-22. doi: 10.1128/AEM.00681-09. Epub 2009 May 15.
2
Mutations in rsmG, encoding a 16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3(2).
J Bacteriol. 2007 May;189(10):3876-83. doi: 10.1128/JB.01776-06. Epub 2007 Mar 23.
3
A key developmental regulator controls the synthesis of the antibiotic erythromycin in Saccharopolyspora erythraea.
Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11346-51. doi: 10.1073/pnas.0803622105. Epub 2008 Aug 6.
4
Polyketide Starter and Extender Units Serve as Regulatory Ligands to Coordinate the Biosynthesis of Antibiotics in Actinomycetes.
mBio. 2021 Oct 26;12(5):e0229821. doi: 10.1128/mBio.02298-21. Epub 2021 Sep 28.
5
Genetically engineered rpsL merodiploidy impacts secondary metabolism and antibiotic resistance in Streptomyces.
World J Microbiol Biotechnol. 2021 Mar 17;37(4):62. doi: 10.1007/s11274-021-03030-5.
6
An aberrant protein synthesis activity is linked with antibiotic overproduction in rpsL mutants of Streptomyces coelicolor A3(2).
Microbiology (Reading). 2003 Nov;149(Pt 11):3299-3309. doi: 10.1099/mic.0.26490-0.
7
Development of antibiotic-overproducing strains by site-directed mutagenesis of the rpsL gene in Streptomyces lividans.
Appl Environ Microbiol. 2003 Jul;69(7):4256-9. doi: 10.1128/AEM.69.7.4256-4259.2003.
8
Two amino acids missing of MtrA resulted in increased erythromycin level and altered phenotypes in Saccharopolyspora erythraea.
Appl Microbiol Biotechnol. 2019 Jun;103(11):4539-4548. doi: 10.1007/s00253-019-09825-9. Epub 2019 Apr 17.
9
Effect of "ribosome engineering" on the transcription level and production of S. albus indigenous secondary metabolites.
Appl Microbiol Biotechnol. 2019 Sep;103(17):7097-7110. doi: 10.1007/s00253-019-10005-y. Epub 2019 Jul 19.
10
Reverse engineering of industrial pharmaceutical-producing actinomycete strains using DNA microarrays.
Metab Eng. 2004 Jul;6(3):186-96. doi: 10.1016/j.ymben.2003.12.001.
引用本文的文献
1
Ribosome Engineering for Enhanced Butenyl-Spinosyn Production in Saccharopolyspora pogona.
Curr Microbiol. 2025 Jun 17;82(8):337. doi: 10.1007/s00284-025-04317-8.
2
Introduction of Spontaneous Mutations Using Streptomycin as a Method for Lactic Acid Bacteria Breeding.
Methods Mol Biol. 2024;2851:15-21. doi: 10.1007/978-1-0716-4096-8_2.
3
Harnessing regulatory networks in Actinobacteria for natural product discovery.
J Ind Microbiol Biotechnol. 2024 Jan 9;51. doi: 10.1093/jimb/kuae011.
4
Combined transcriptomic and pangenomic analyses guide metabolic amelioration to enhance tiancimycins production.
Appl Microbiol Biotechnol. 2024 Dec;108(1):18. doi: 10.1007/s00253-023-12937-y. Epub 2024 Jan 3.
5
Small molecule inducers of actinobacteria natural product biosynthesis.
J Ind Microbiol Biotechnol. 2023 Feb 17;50(1). doi: 10.1093/jimb/kuad019.
6
Acquisition of Streptomycin Resistance by Oxidative Stress Induced by Hydrogen Peroxide in Radiation-Resistant Bacterium .
Int J Mol Sci. 2022 Aug 28;23(17):9764. doi: 10.3390/ijms23179764.
7
Properties of Spontaneous Mutant of KO-1297.
Cytol Genet. 2022;56(1):31-36. doi: 10.3103/S009545272201011X. Epub 2022 Feb 11.
8
Specialized Metabolites from Ribosome Engineered Strains of .
Metabolites. 2021 Apr 13;11(4):239. doi: 10.3390/metabo11040239.
9
Genetically engineered rpsL merodiploidy impacts secondary metabolism and antibiotic resistance in Streptomyces.
World J Microbiol Biotechnol. 2021 Mar 17;37(4):62. doi: 10.1007/s11274-021-03030-5.
10
Substantial improvement of tetraene macrolide production in Streptomyces diastatochromogenes by cumulative drug resistance mutations.
PLoS One. 2020 May 12;15(5):e0232927. doi: 10.1371/journal.pone.0232927. eCollection 2020.
本文引用的文献
1
Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12.
Nat Biotechnol. 2009 May;27(5):462-4. doi: 10.1038/nbt.1538. Epub 2009 Apr 26.
2
Phenotypes and gene expression profiles of Saccharopolyspora erythraea rifampicin-resistant (rif) mutants affected in erythromycin production.
Microb Cell Fact. 2009 Mar 30;8:18. doi: 10.1186/1475-2859-8-18.
3
Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis.
Appl Microbiol Biotechnol. 2009 Apr;82(6):1089-96. doi: 10.1007/s00253-008-1850-2. Epub 2009 Jan 16.
4
A novel insertion mutation in Streptomyces coelicolor ribosomal S12 protein results in paromomycin resistance and antibiotic overproduction.
Antimicrob Agents Chemother. 2009 Mar;53(3):1019-26. doi: 10.1128/AAC.00388-08. Epub 2008 Dec 22.
5
Activation of dormant bacterial genes by Nonomuraea sp. strain ATCC 39727 mutant-type RNA polymerase.
J Bacteriol. 2009 Feb;191(3):805-14. doi: 10.1128/JB.01311-08. Epub 2008 Dec 1.
6
A key developmental regulator controls the synthesis of the antibiotic erythromycin in Saccharopolyspora erythraea.
Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11346-51. doi: 10.1073/pnas.0803622105. Epub 2008 Aug 6.
7
Feast or famine: the global regulator DasR links nutrient stress to antibiotic production by Streptomyces.
EMBO Rep. 2008 Jul;9(7):670-5. doi: 10.1038/embor.2008.83. Epub 2008 May 30.
8
Dramatic activation of antibiotic production in Streptomyces coelicolor by cumulative drug resistance mutations.
Appl Environ Microbiol. 2008 May;74(9):2834-40. doi: 10.1128/AEM.02800-07. Epub 2008 Feb 29.
9
From microbial differentiation to ribosome engineering.
Biosci Biotechnol Biochem. 2007 Jun;71(6):1373-86. doi: 10.1271/bbb.70007.
10
Mutations in rsmG, encoding a 16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3(2).
J Bacteriol. 2007 May;189(10):3876-83. doi: 10.1128/JB.01776-06. Epub 2007 Mar 23.
Zotero 插件