Young R B, Bergen W G, Blauwiekel P B
J Cell Biol. 1979 Apr;81(1):115-22. doi: 10.1083/jcb.81.1.115.
The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2 x 10(3) molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half-life. This calculated rate of approximately 2.9 x 10(3) molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was approximately 2.9 x 10(4) molecules/nucleus/min.
通过用[³H]亮氨酸脉冲标记，在复制的胚胎鸡皮肤成纤维细胞培养物中测量组成型肌球蛋白的合成速率。这些细胞以3.2×10³个分子/细胞/分钟的速率合成200,000道尔顿的肌球蛋白重链（MHC）。此外，根据总蛋白含量、MHC百分比、成纤维细胞倍增时间和MHC半衰期，计算出维持每个细胞中组成型MHC恒定水平所需的MHC合成速率的独立估计值。这个计算出的速率约为2.9×10³个分子/细胞/分钟，与测量速率非常一致。相比之下，在完全活化的肌肉细胞培养物中，肌原纤维MHC的合成速率约为2.9×10⁴个分子/细胞核/分钟。
