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胚胎鸡成纤维细胞中的肌球蛋白合成

Myosin synthesis in embryonic chicken fibroblasts.

作者信息

Young R B, Bergen W G, Blauwiekel P B

出版信息

J Cell Biol. 1979 Apr;81(1):115-22. doi: 10.1083/jcb.81.1.115.

DOI:10.1083/jcb.81.1.115
PMID:479285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2111524/
Abstract

The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2 x 10(3) molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half-life. This calculated rate of approximately 2.9 x 10(3) molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was approximately 2.9 x 10(4) molecules/nucleus/min.

摘要

通过用[³H]亮氨酸脉冲标记，在复制的胚胎鸡皮肤成纤维细胞培养物中测量组成型肌球蛋白的合成速率。这些细胞以3.2×10³个分子/细胞/分钟的速率合成200,000道尔顿的肌球蛋白重链（MHC）。此外，根据总蛋白含量、MHC百分比、成纤维细胞倍增时间和MHC半衰期，计算出维持每个细胞中组成型MHC恒定水平所需的MHC合成速率的独立估计值。这个计算出的速率约为2.9×10³个分子/细胞/分钟，与测量速率非常一致。相比之下，在完全活化的肌肉细胞培养物中，肌原纤维MHC的合成速率约为2.9×10⁴个分子/细胞核/分钟。

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本文引用的文献

1
An analysis of myogenesis by the use of fluorescent antimyosin.利用荧光抗肌球蛋白对肌发生进行的分析。
J Biophys Biochem Cytol. 1957 Sep 25;3(5):705-24. doi: 10.1083/jcb.3.5.705.
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Cytoplasmic filaments of Amoeba proteus. I. The role of filaments in consistency changes and movement.变形虫的细胞质细丝。I. 细丝在稠度变化和运动中的作用。
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Isolation and characterization of myosin from cloned mouse fibroblasts.从小鼠克隆成纤维细胞中分离和鉴定肌球蛋白。
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The modification of actomyosin by alpha- actinin. I. A survey of experimental conditions.
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Actin antibody: the specific visualization of actin filaments in non-muscle cells.肌动蛋白抗体：非肌肉细胞中肌动蛋白丝的特异性可视化。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268-72. doi: 10.1073/pnas.71.6.2268.
7
Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells.多种成肌细胞和非成肌细胞中肌动蛋白和肌球蛋白的协同合成与降解。
Exp Cell Res. 1976 Feb;97(2):387-93. doi: 10.1016/0014-4827(76)90630-3.
8
Isolation of myosin-synthesizing polysomes from cultures of embryonic chicken myoblasts before fusion.在胚胎鸡成肌细胞融合前的培养物中分离合成肌球蛋白的多核糖体。
Dev Biol. 1975 Nov;47(1):123-35. doi: 10.1016/0012-1606(75)90268-7.
9
A mechanical dissociation method for preparation of muscle cell cultures.一种用于制备肌肉细胞培养物的机械解离方法。
J Cell Physiol. 1975 Dec;86(3 Pt 1):561-5. doi: 10.1002/jcp.1040860313.
10
Non-identity of muscle and non-muscle actins.肌肉肌动蛋白与非肌肉肌动蛋白的非同一性。
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