Tepperman K, Morris G, Essien F, Heywood S M
J Cell Physiol. 1975 Dec;86(3 Pt 1):561-5. doi: 10.1002/jcp.1040860313.
A cell preparation method by which large numbers of embryonic chick skeletal muscle cells may be obtained is described. The procedure requires fewer manipulations and much less time than standard trypsinization. By the criteria used, both methods are comparable with respect to percent viable cells and survival of plated cells. However, in addition to the ease of preparation, the mechanical dissociation method offers the significant advantage that the cell suspension is greatly enriched for myoblasts without the necessity of an additional preplating step.
本文描述了一种可获取大量鸡胚骨骼肌细胞的细胞制备方法。与标准胰蛋白酶消化法相比，该方法所需操作更少，耗时更短。根据所采用的标准，两种方法在活细胞百分比和接种细胞存活率方面相当。然而，除了制备简便之外，机械解离法还有一个显著优点，即细胞悬液中肌母细胞大量富集，无需额外的预铺板步骤。
