正常和转化大鼠肾细胞中微管蛋白的组织
Organization of tubulin in normal and transformed rat kidney cells.
作者信息
Rubin R W, Warren R H
出版信息
J Cell Biol. 1979 Jul;82(1):103-13. doi: 10.1083/jcb.82.1.103.
We have carried out a quantitative biochemical and ultrastructural study of tubulin and microtubules in a normal rat kidney (NRK) cell line and its viral transformant (442) in culture. Under equivalent culture conditions, both cell lines contain the same amount of tubulin according to a colchicine-binding assay. The normal and transformed cells differ significantly, however, with respect to the state of organization of their tubulin. Counts of microtubules in sectioned cells indicate that NRK cells have almost twice as many microtubules per unit area of cytoplasm as the 442 cells. Centrifugation studies, on the other hand, show that 442 cells have almost twice as much pelletable tubulin as the NRK cells. We propose, therefore, that the transformed cells contain a large amount of tubulin which is in some alternative aggregate form that is not morphologically detectable as microtubles in the cytoplasm
我们对正常大鼠肾（NRK）细胞系及其培养中的病毒转化细胞（442）进行了微管蛋白和微管的定量生化及超微结构研究。在相同的培养条件下，根据秋水仙碱结合试验，两种细胞系所含微管蛋白的量相同。然而，正常细胞和转化细胞在微管蛋白的组织状态方面存在显著差异。对切片细胞中的微管进行计数表明，NRK细胞每单位细胞质面积中的微管数量几乎是442细胞的两倍。另一方面，离心研究表明，442细胞中可沉淀的微管蛋白量几乎是NRK细胞的两倍。因此，我们提出，转化细胞含有大量微管蛋白，这些微管蛋白以某种其他聚集形式存在，在细胞质中无法通过形态学检测到微管。
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