De Mey J, Joniau M, De Brabander M, Moens W, Geuens G
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1339-43. doi: 10.1073/pnas.75.3.1339.
By using immunoperoxidase cytochemistry at the light and electron microscopic level, microtubles were visualized in a number of "normal" nontumorigenic and transformed tumorigenic cell lines. A well-defined cytoplasmic microtubule complex exists in both normal and transformed interphase cells. The distribution of this complex closely correlates with the cell shape and the degree of cell spreading. Our data support the idea that these properties determine the pattern of the cytoplasmic microtubule complex, rather than the reverse. Ultrastructural observations of immunoperoxidase-stained tumor cells showed characteristic microtubules in cells in which the microtubules were poorly resolved at the light microscopic level. The results suggest that microtubule assembly and structure are unaltered in transformed cells. However, this conclusion does not exclude the possibility that some of the microtubules' functions might be impaired in a yet-unknown way.
通过在光镜和电镜水平上使用免疫过氧化物酶细胞化学方法，在许多“正常”非致瘤性和转化的致瘤性细胞系中观察到了微管。在正常和转化的间期细胞中均存在明确的细胞质微管复合体。该复合体的分布与细胞形状和细胞铺展程度密切相关。我们的数据支持这样一种观点，即这些特性决定了细胞质微管复合体的模式，而非相反。对免疫过氧化物酶染色的肿瘤细胞的超微结构观察显示，在光镜水平上微管分辨不清的细胞中存在特征性微管。结果表明，转化细胞中的微管组装和结构未发生改变。然而，这一结论并不排除某些微管功能可能以未知方式受损的可能性。
