豚鼠气管中去甲肾上腺素细胞外积聚的组织化学研究。

Accumulation of noradrenaline in extraneuronal tissue of the guinea-pig trachea has been studied by the use of the fluorescence histochemical technique of Falck and Hillarp.2. After incubation in solutions of noradrenaline, fluorescence developed in cellular structures (tracheal smooth muscle, vascular smooth muscle and endothelium, fibroblasts and chondroblasts), in the intercellular matrix of the cartilage and throughout the loose connective tissue of the adventitia and submucosa.3. The effect of various experimental procedures on the development of this fluorescence has been examined i.e. incubation with noradrenaline at reduced temperature (0 degrees C), removal of fluorescence by washing with Krebs solution at 37 degrees C or 0 degrees C, incubation with phenoxybenzamine (10(-4)M) or metanephrine (10(-4)M).4. From these observations it has been concluded that noradrenaline accumulates in the trachea:(a) in cellular structures where it is firmly bound, i.e. not easily removed by washing at 0 degrees C, and where the accumulation is prevented by phenoxybenzamine, metanephrine or cold.(b) in the intercellular matrix of the cartilage where it is also firmly bound, but where the accumulation is not prevented by phenoxybenzamine, metanephrine or cold. This probably represents binding to sulphated mucopolysaccharides.(c) in the adventitia and submucosa where it is loosely bound and easily removed by washing.5. Some implications of these findings in pharmacological experiments with guinea-pig trachea are discussed.

运用法尔克和希拉尔普的荧光组织化学技术，对豚鼠气管神经外组织中去甲肾上腺素的蓄积情况进行了研究。
在去甲肾上腺素溶液中孵育后，细胞结构（气管平滑肌、血管平滑肌和内皮、成纤维细胞和成软骨细胞）、软骨的细胞间基质以及外膜和黏膜下层的疏松结缔组织中均出现了荧光。
已检测了各种实验操作对这种荧光产生的影响，即：在低温（0摄氏度）下与去甲肾上腺素孵育、在37摄氏度或0摄氏度用克雷布斯溶液冲洗以去除荧光、与苯氧苄胺（10⁻⁴M）或间甲肾上腺素（10⁻⁴M）孵育。
从这些观察结果得出结论，去甲肾上腺素在气管中蓄积于：

(a) 细胞结构中，在其中它紧密结合，即不易在0摄氏度冲洗去除，且苯氧苄胺、间甲肾上腺素或低温可阻止其蓄积。

(b) 软骨的细胞间基质中，在其中它同样紧密结合，但苯氧苄胺、间甲肾上腺素或低温不能阻止其蓄积。这可能代表与硫酸化粘多糖的结合。