Capron F, Coltoff-Schiller B, Johnson A B, Fleischner G M, Goldfischer S
J Histochem Cytochem. 1979 May;27(5):961-6. doi: 10.1177/27.5.479557.
Ligandin (glutathione-s-transferase) and Z protein are soluble hepatocellular proteins that are involved in the transfer of organic ions, including bilirubin and some hormones and carcinogens from the plasma to the liver. The intracellular distribution of ligandin and Z protein was studied by applying the peroxidase-antiperoxidase procedure of L. A. Sternberger (Immunocytochemistry, Prentice Hall Inc., 1974) to paraffin sections and free-floating 10-micrometers frozen sections that were processed for both light and electron microscopy. Ligandin and Z protein were localized to the cytosol of hepatocytes in association with smooth endoplasmic reticulum (SER), but no reaction product was present between cisternae of rough endoplasmic reticulum. Penetration of reagents was enhanced in 10-micrometers frozen sections and the preservation of subcellular structures was equivalent to thicker, unfrozen sections.
配体蛋白（谷胱甘肽 - S - 转移酶）和Z蛋白是可溶性肝细胞蛋白，参与包括胆红素、一些激素和致癌物在内的有机离子从血浆到肝脏的转运。通过将L. A. 斯特恩伯格的过氧化物酶 - 抗过氧化物酶方法（《免疫细胞化学》，普伦蒂斯·霍尔公司，1974年）应用于石蜡切片和用于光镜及电镜观察的10微米厚的漂浮冰冻切片，研究了配体蛋白和Z蛋白在细胞内的分布。配体蛋白和Z蛋白定位于肝细胞的胞质溶胶中，与滑面内质网（SER）相关，但粗面内质网的池之间没有反应产物。在10微米厚的冰冻切片中，试剂的穿透力增强，亚细胞结构的保存与较厚的未冰冻切片相当。
