Owen C S, Winger L A, Symington F W, Nowell P C
J Immunol. 1979 Oct;123(4):1778-80.
High gradient magnetic separation, which as previously been shown effective in extracting erythrocytes from a flowing cell suspension, has been used to separate rosetted and unrosetted human peripheral blood lymphocytes. The hemoglobin in the sheep red cells used to form rosettes was first oxidizied to the paramagnetic methemoglobin form. Samples of 50 x 10(6) lymphocytes could be processed in 10 min under sterile conditions with greater than 90% purity of the rosetted cell fraction and maintenance of T cell function in mixed lymphocyte cultures.
高梯度磁分离先前已被证明可有效地从流动的细胞悬液中提取红细胞，现被用于分离形成玫瑰花结和未形成玫瑰花结的人外周血淋巴细胞。用于形成玫瑰花结的绵羊红细胞中的血红蛋白首先被氧化为顺磁性高铁血红蛋白形式。在无菌条件下，50×10⁶个淋巴细胞的样本可在10分钟内处理完毕，形成玫瑰花结的细胞组分纯度大于90%，且在混合淋巴细胞培养中T细胞功能得以维持。
