Brown R A, Potts R C, Robertson A J, Hayes P C, Ramesar K, Beck J S
J Immunol Methods. 1979;29(2):117-31. doi: 10.1016/0022-1759(79)90061-9.
Visual counts of the number of SRBC attached to individual lymphocytes in E-rosette preparations from 3 healthy young adults were made by 3 experienced observers: from these data the size distribution profile of particles in each E-rosette preparation was calculated. In parallel experiments the size distribution profile was measured directly with a particle size analyser (Coulter Counter and Channelyzer): in every case the observed profile lay within the 95% confidence band around the reconstructed profiles. It was concluded that the Coulter Channelyzer profile is an accurate measurement of the E-rosette size distribution and that particle sizing could provide a reasonable basis for semi-automated tests for E-rosettes. A mathematical model has been developed by which the percentage of lymphocytes forming E-rosettes, the distribution of number of attached SRBC and the average avidity of the lymphocyte receptor for SRBC could be deduced from the size-distribution profiles. The method has a high degree of precision, and a good correlation (P less than 0.01) was obtained when the percentage of E-rosettes (defined for this purpose as having three or more attached SRBC) obtained from the new method was compared with results from standard light microscopy.
3名经验丰富的观察者对来自3名健康年轻成年人的E花环制备物中附着于单个淋巴细胞的SRBC数量进行了目视计数：根据这些数据计算出每个E花环制备物中颗粒的大小分布概况。在平行实验中，使用粒度分析仪（库尔特计数器和通道分析仪）直接测量大小分布概况：在每种情况下，观察到的概况都落在围绕重建概况的95%置信带内。得出的结论是，库尔特通道分析仪概况是对E花环大小分布的准确测量，并且颗粒大小测定可为E花环的半自动检测提供合理依据。已经开发出一种数学模型，通过该模型可以从大小分布概况中推导出形成E花环的淋巴细胞百分比、附着的SRBC数量分布以及淋巴细胞受体对SRBC的平均亲和力。该方法具有高度的精确性，当将新方法获得的E花环百分比（为此定义为具有三个或更多附着的SRBC）与标准光学显微镜的结果进行比较时，获得了良好的相关性（P小于0.01）。
