金黄色葡萄球菌肠毒素A和B：食品中的固相放射免疫测定法
Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.
作者信息
Johnson H M, Bukovic J A, Kauffmann P E
出版信息
Appl Microbiol. 1973 Sep;26(3):309-13. doi: 10.1128/am.26.3.309-313.1973.
Abstract
An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per g of food; nonspecific inhibitions were 15% or less.
摘要
采用碘-125标记的A、B型肠毒素以及包被有特异性抗体的聚苯乙烯试管进行免疫分析，用于检测和定量食品中的肠毒素。研究了火腿沙拉、切达干酪、蛋奶冻、炼乳和意大利腊肠。通过简单的提取程序成功测定了所有食品中的肠毒素。该分析对每克食品中1至10纳克的毒素敏感；非特异性抑制率为15%或更低。
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