以含有A蛋白的葡萄球菌细胞作为免疫吸附剂，通过放射免疫分析法检测食品中的葡萄球菌肠毒素A、B、C、D和E。
Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent.
作者信息
Miller B A, Reiser R F, Bergdoll M S
出版信息
Appl Environ Microbiol. 1978 Sep;36(3):421-6. doi: 10.1128/aem.36.3.421-426.1978.
Abstract
A sensitive radioimmunoassay utilizing Staphylococcus aureus cells containing protein A as a coprecipitant was developed for the detection and quantitation of staphylococcal enterotoxins A, B, C, D, and E in a variety of foods. The enterotoxins were extracted from the foods by a simple and rapid procedure. The sensitivity of the assay is 1.0 ng or less of enterotoxin per g of food.
摘要
开发了一种灵敏的放射免疫测定法，该方法利用含有蛋白A的金黄色葡萄球菌细胞作为共沉淀剂，用于检测和定量多种食品中的葡萄球菌肠毒素A、B、C、D和E。通过简单快速的程序从食品中提取肠毒素。该测定法的灵敏度为每克食品中肠毒素1.0纳克或更少。
相似文献
1
Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent.以含有A蛋白的葡萄球菌细胞作为免疫吸附剂，通过放射免疫分析法检测食品中的葡萄球菌肠毒素A、B、C、D和E。
Appl Environ Microbiol. 1978 Sep;36(3):421-6. doi: 10.1128/aem.36.3.421-426.1978.
2
Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.金黄色葡萄球菌肠毒素A和B：食品中的固相放射免疫测定法
Appl Microbiol. 1973 Sep;26(3):309-13. doi: 10.1128/am.26.3.309-313.1973.
3
The use of polyethylene glycol in radioimmunoassay of staphylococcal enterotoxins.
Can J Microbiol. 1978 Jun;24(6):765-6. doi: 10.1139/m78-128.
4
Improved radioimmunoassay of Staphylococcal enterotoxin a.
J Assoc Off Anal Chem. 1982 Jan;65(1):180-4.
5
Double-antibody radioimmunoassay for staphylococcal enterotoxin C2.葡萄球菌肠毒素C2的双抗体放射免疫测定法
Appl Microbiol. 1975 Oct;30(4):525-9. doi: 10.1128/am.30.4.525-529.1975.
6
Double-antibody radioimmunoassay for staphylococcal enterotoxins A and B.
Can J Microbiol. 1978 Apr;24(4):436-9. doi: 10.1139/m78-072.
7
Staphylococcal enterotoxin C: solid-phase radioimmunoassay.葡萄球菌肠毒素C：固相放射免疫测定法
Appl Microbiol. 1975 Oct;30(4):700-1. doi: 10.1128/am.30.4.700-701.1975.
8
Evaluation of radioimmunoassay performance for staphylococcal enterotoxin in foods: application of external quality assessment survey.
Can J Microbiol. 1986 Jul;32(7):602-4. doi: 10.1139/m86-111.
9
Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods.用于检测食品中葡萄球菌肠毒素的酶联免疫吸附测定法。
Appl Environ Microbiol. 1982 Dec;44(6):1349-55. doi: 10.1128/aem.44.6.1349-1355.1982.
10
Statistical analysis and quality control in radioimmunoassays for staphylococcal enterotoxins A, B, and C.葡萄球菌肠毒素A、B和C放射免疫分析中的统计分析与质量控制
Appl Environ Microbiol. 1977 Dec;34(6):710-4. doi: 10.1128/aem.34.6.710-714.1977.
引用本文的文献
1
16 Methods for Detecting Microbial Pathogens in Food and Water.16种食品和水中微生物病原体的检测方法。
Methods Microbiol. 1990;22:497-531. doi: 10.1016/S0580-9517(08)70253-8. Epub 2008 Apr 28.
2
Staphylococcal enterotoxin production in the presence of non-enterotoxigenic staphylococci.在非产肠毒素葡萄球菌存在的情况下葡萄球菌肠毒素的产生
Appl Environ Microbiol. 1980 Jun;39(6):1167-71. doi: 10.1128/aem.39.6.1167-1171.1980.
3
Determination of staphylococcal enterotoxin A in cheddar cheese produced without starter activity.在无发酵剂活性条件下生产的切达干酪中葡萄球菌肠毒素A的测定。
Appl Environ Microbiol. 1980 Jun;39(6):1134-7. doi: 10.1128/aem.39.6.1134-1137.1980.
4
Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods.用于检测食品中葡萄球菌肠毒素的酶联免疫吸附测定法。
Appl Environ Microbiol. 1982 Dec;44(6):1349-55. doi: 10.1128/aem.44.6.1349-1355.1982.
5
Direct skin test in highly sensitized guinea pigs for rapid and sensitive determination of staphylococcal enterotoxin B.高敏豚鼠的直接皮肤试验用于快速灵敏地测定葡萄球菌肠毒素B
Appl Environ Microbiol. 1983 Dec;46(6):1351-6. doi: 10.1128/aem.46.6.1351-1356.1983.
6
Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.葡萄球菌肠毒素C3的鉴定、纯化及某些理化性质
Infect Immun. 1984 Sep;45(3):625-30. doi: 10.1128/iai.45.3.625-630.1984.
7
Effects of staphylococcal enterotoxin A on the rat gastrointestinal tract.葡萄球菌肠毒素A对大鼠胃肠道的影响。
Infect Immun. 1984 May;44(2):234-40. doi: 10.1128/iai.44.2.234-240.1984.
8
Monoclonal antibodies to staphylococcal enterotoxins B and C: cross-reactivity and localization of epitopes on tryptic fragments.抗葡萄球菌肠毒素B和C的单克隆抗体：表位在胰蛋白酶消化片段上的交叉反应性及定位
Infect Immun. 1984 Jul;45(1):281-5. doi: 10.1128/iai.45.1.281-285.1984.
9
Detection of staphylococcal enterotoxins by enzyme-linked immunosorbent assays and radioimmunoassays: comparison of monoclonal and polyclonal antibody systems.通过酶联免疫吸附测定法和放射免疫测定法检测葡萄球菌肠毒素：单克隆和多克隆抗体系统的比较
Appl Environ Microbiol. 1986 May;51(5):885-90. doi: 10.1128/aem.51.5.885-890.1986.
10
Incidence of antibodies reactive with toxic shock syndrome toxin 1 in bovine milk.牛乳中与中毒性休克综合征毒素1反应的抗体发生率。
Appl Environ Microbiol. 1986 Apr;51(4):865-7. doi: 10.1128/aem.51.4.865-867.1986.
本文引用的文献
1
Modified radioimmunoassay determination for staphylococcal enterotoxin B in foods.改良放射免疫分析法测定食品中的葡萄球菌肠毒素 B。
Appl Environ Microbiol. 1977 Mar;33(3):620-5. doi: 10.1128/aem.33.3.620-625.1977.
2
DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.食品中葡萄球菌肠毒素的检测
Appl Microbiol. 1965 Mar;13(2):181-9. doi: 10.1128/am.13.2.181-189.1965.
3
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
4
Insulin-I131 metabolism in human subjects: demonstration of insulin binding globulin in the circulation of insulin treated subjects.人类受试者中胰岛素-I131的代谢：胰岛素治疗受试者循环中胰岛素结合球蛋白的证实。
J Clin Invest. 1956 Feb;35(2):170-90. doi: 10.1172/JCI103262.
5
Enzymatic iodination of polypeptides with 125I to high specific activity.用¹²⁵I对多肽进行酶促碘化以获得高比活度。
Biochim Biophys Acta. 1971 Dec 28;251(3):363-9. doi: 10.1016/0005-2795(71)90123-1.
6
Staphylococcal enterotoxin B: solid-phase radioimmunoassay.葡萄球菌肠毒素B：固相放射免疫测定法
Appl Microbiol. 1971 Nov;22(5):837-41. doi: 10.1128/am.22.5.837-841.1971.
7
Ammonium sulfate coprecipitation antibody determination with purified staphylococcal enterotoxins.用纯化的葡萄球菌肠毒素进行硫酸铵共沉淀抗体测定。
J Bacteriol. 1969 Jul;99(1):18-24. doi: 10.1128/jb.99.1.18-24.1969.
8
Detection of staphylococcal enterotoxin in foods.食品中葡萄球菌肠毒素的检测
Appl Microbiol. 1974 Jan;27(1):83-5. doi: 10.1128/am.27.1.83-85.1974.
9
Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.金黄色葡萄球菌肠毒素A和B：食品中的固相放射免疫测定法
Appl Microbiol. 1973 Sep;26(3):309-13. doi: 10.1128/am.26.3.309-313.1973.
10
Rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A.葡萄球菌肠毒素A的快速固相放射免疫测定法。
Appl Microbiol. 1973 May;25(5):774-7. doi: 10.1128/am.25.5.774-777.1973.
Zotero 插件