小鼠IgG2b与小鼠巨噬细胞上Fc受体的结合位点。
Site of binding of mouse IgG2b to the Fc receptor on mouse macrophages.
作者信息
Diamond B, Birshtein B K, Scharff M D
出版信息
J Exp Med. 1979 Sep 19;150(3):721-6. doi: 10.1084/jem.150.3.721.
Three mouse immunoglobulins with altered heavy chains have been used to study the specificity of the mouse IgG2b Fc receptor on mouse macrophages. These immunoglobulins were synthesized by variant clones derived from the MPC 11, IgG2b-producing mouse myeloma cell line. One variant, whose Fc receptor. A second variant, which makes a short heavy chain lacking the CH3 domain, binds specifically to the IgG2b Fc receptor. The third variant makes a hybrid IgG2b-IgG2a heavy chain whose CH3 domain is enterely IgG2a-like and binds to both IgG2a and IgG2b Fc receptors. These data suggest that the binding of mouse IgG2b immunoglobulins to the mouse macrophage Fc receptor involves a site within the CH2 domain and indicate that immunoglobulins with altered heavy chains are a useful tool to probe Fc receptors.
三种重链发生改变的小鼠免疫球蛋白已被用于研究小鼠巨噬细胞上小鼠IgG2b Fc受体的特异性。这些免疫球蛋白由源自产生IgG2b的小鼠骨髓瘤细胞系MPC 11的变异克隆合成。一种变异体，其Fc受体……。第二种变异体产生一种缺少CH3结构域的短重链，它特异性地结合IgG2b Fc受体。第三种变异体产生一种杂交的IgG2b-IgG2a重链，其CH3结构域完全类似IgG2a，能结合IgG2a和IgG2b Fc受体。这些数据表明小鼠IgG2b免疫球蛋白与小鼠巨噬细胞Fc受体的结合涉及CH2结构域内的一个位点，并表明重链发生改变的免疫球蛋白是探测Fc受体的有用工具。
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