Jenner W N, Rose P A
Biochem J. 1973 Sep;135(1):109-14. doi: 10.1042/bj1350109.
The formation of sulpho-conjugates of 3,4-dihydroxyphenylethylamine (dopamine) and related compounds was examined in preparations of rat tissues. Liver high-speed-supernatant preparations readily transferred sulphate from adenosine 3'-phosphate 5'-sulphato-phosphate to dopamine under standard conditions. The main product was identified as the 3-O-sulphate. The preparation also sulphated the 3- and 4-methoxy derivatives but to a lesser extent (44% and 95% respectively) relative to dopamine. Brain preparations possessed only half the activity of liver but formed both the 3- and 4-O-sulphates in the molar ratio of 1.7:1. l-3,4-Dihydroxyphenylalanine (l-dopa) in both tissue preparations did not yield any significant amount of sulpho-conjugate when the dopa decarboxylase present was inhibited. The sulphotransferase activity of preparations was doubled in the presence of dithiothreitol and it was concluded that l-tyrosine methyl ester sulphotransferase was the enzyme involved. A method for the preparation of authentic dopamine 3-O-sulphate and 4-O-sulphate was developed.
在大鼠组织制剂中研究了3,4 - 二羟基苯乙胺（多巴胺）及相关化合物的硫酸结合物的形成。在标准条件下，肝脏高速上清液制剂能轻易地将硫酸根从3'-磷酸腺苷5'-硫酸磷酸转移至多巴胺。主要产物被鉴定为3 - O - 硫酸盐。该制剂也能使3 - 和4 - 甲氧基衍生物硫酸化，但相对于多巴胺程度较低（分别为44%和95%）。脑制剂的活性仅为肝脏的一半，但能以1.7:1的摩尔比形成3 - 和4 - O - 硫酸盐。当存在的多巴脱羧酶被抑制时，两种组织制剂中的L - 3,4 - 二羟基苯丙氨酸（L - 多巴）均未产生任何显著量的硫酸结合物。在二硫苏糖醇存在下，制剂的磺基转移酶活性增加一倍，得出结论认为L - 酪氨酸甲酯磺基转移酶是相关酶。开发了一种制备纯多巴胺3 - O - 硫酸盐和4 - O - 硫酸盐的方法。
