高酪氨酸含量对酸性茚三酮法测定蛋白质中色氨酸的影响。应用于鸡卵类粘蛋白。
Effect of high tyrosine content on the determination of tryptophan in protein by the acidic ninhydrin method. Application to chicken ovoinhibitor.
作者信息
Zahnley J C, Davis J G
出版信息
Biochem J. 1973 Sep;135(1):59-61. doi: 10.1042/bj1350059.
Abstract
Colorimetric determination of tryptophan in intact proteins by the acidic ninhydrin method of Gaitonde & Dovey (1970) gives high apparent tryptophan contents for proteins having high tyrosine/tryptophan ratios. Correction for this interference by tyrosine can be achieved by plotting the ratio of observed to expected tryptophan content as a function of tyrosine/tryptophan ratio for proteins of known composition. The equation of the line is: [Formula: see text] Application of this correction to chicken ovoinhibitor, which contains 17 tyrosine residues per molecule, gave results that agree with tryptophan content determined by other methods.
摘要
采用盖顿德和多韦（1970年）的酸性茚三酮法对完整蛋白质中的色氨酸进行比色测定时，对于酪氨酸/色氨酸比率较高的蛋白质，测得的色氨酸含量明显偏高。对于酪氨酸造成的这种干扰，可以通过将观察到的色氨酸含量与预期色氨酸含量之比，绘制为已知组成蛋白质的酪氨酸/色氨酸比率的函数，来进行校正。该直线方程为：[公式：见原文] 将此校正方法应用于每个分子含有17个酪氨酸残基的鸡卵清抑制剂时，得到的结果与通过其他方法测定的色氨酸含量相符。
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本文引用的文献
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Physical characterization of ovoinhibitor, a trypsin and chymotrypsin inhibitor from chicken egg white.卵类黏蛋白的物理特性，一种来自鸡蛋白的胰蛋白酶和糜蛋白酶抑制剂。
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Determiniation of trypsin-inhibitor complex dissociation by use of the active site titrant, p-nitrophenyl p'-guanidinobenzoate.使用活性位点滴定剂对硝基苯基对'-胍基苯甲酸酯测定胰蛋白酶抑制剂复合物的解离
Biochemistry. 1970 Mar 17;9(6):1428-33. doi: 10.1021/bi00808a018.
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A rapid and direct method for the quantitative determination of tryptophan in the intact protein.一种用于定量测定完整蛋白质中色氨酸的快速直接方法。
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Methods of tryptophan analysis.色氨酸分析方法。
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