基于荧光光谱法中内滤光效应测定天然蛋白质中的色氨酸和酪氨酸。
The determination of tryptophan and tyrosine in native proteins on the basis of inner filter effect in spectrofluorimetry.
作者信息
Jankowski A, Siemion I Z
出版信息
Acta Biochim Pol. 1977;24(1):13-20.
PMID:868434
Abstract
Determination of tryptophan content in proteins, or tyrosine in proteins not containing tryptophan, is described. The method was applied to five proteins containing from 2 to 8 tryptophan residues per molecule. It was found that the simultaneous presence of a large amount of tyrosine in the protein has no influence on the accuracy of tryptophan determination.
摘要
本文描述了蛋白质中色氨酸含量的测定方法，以及不含色氨酸的蛋白质中酪氨酸含量的测定方法。该方法应用于五种每分子含有2至8个色氨酸残基的蛋白质。结果发现，蛋白质中同时存在大量酪氨酸对色氨酸测定的准确性没有影响。
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