White L A
J Clin Microbiol. 1987 Jul;25(7):1221-4. doi: 10.1128/jcm.25.7.1221-1224.1987.
The susceptibility of the C6/36 clone of Aedes albopictus monolayer cell cultures was determined with 46 prototype viruses passed through three subcultures. Viral growth was confirmed by titration of the passage material in other susceptible host systems. Nineteen viruses demonstrated good growth in C6/36 cells: coxsackievirus group A type 10 and group B types 2, 3, 4, and 5; enterovirus 69; mumps virus; poliovirus types 1 to 3; reovirus types 1 to 3; vaccinia virus; dengue virus type 2; eastern equine encephalomyelitis virus; La Crosse virus; Rocio virus; and St. Louis encephalitis virus. Ten viruses did not adapt to growth in the C6/36 cultures. Seventeen other virus strains displayed only limited growth which was primarily restricted to the initial C6/36 passage or was detected by hemagglutinin reactions without observable cell degeneration. Of the 46 viruses, 33 (72%) were capable of initiating infection with a demonstrable cytopathic effect in the initial C6/36 passage. Hemagglutination or complement fixation titers or both were obtained with dengue virus type 2, eastern equine encephalomyelitis virus, La Crosse virus, mumps virus, reovirus types 1 to 3, and Rocio, St. Louis encephalitis, and vaccinia viruses.
用经过三次传代培养的46种原型病毒测定了白纹伊蚊单层细胞培养物C6/36克隆的易感性。通过在其他易感宿主系统中对传代材料进行滴定来确认病毒生长情况。19种病毒在C6/36细胞中生长良好：柯萨奇病毒A组10型以及B组2、3、4和5型；肠道病毒69型；腮腺炎病毒；脊髓灰质炎病毒1至3型；呼肠孤病毒1至3型；痘苗病毒；登革病毒2型；东部马脑炎病毒；拉克罗斯病毒；罗西奥病毒；以及圣路易斯脑炎病毒。10种病毒无法适应在C6/36培养物中生长。另外17种病毒株仅表现出有限的生长，主要局限于最初的C6/36传代，或者通过血凝反应检测到但无明显细胞变性。在这46种病毒中，33种（72%）能够在最初的C6/36传代中引发具有明显细胞病变效应的感染。对登革病毒2型、东部马脑炎病毒、拉克罗斯病毒、腮腺炎病毒、呼肠孤病毒1至3型以及罗西奥、圣路易斯脑炎和痘苗病毒进行了血凝或补体结合滴定或两者都进行了滴定。
