Boone R F, Parr R P, Moss B
J Virol. 1979 Apr;30(1):365-74. doi: 10.1128/JVI.30.1.365-374.1979.
Approximately 15% of the polyadenylic acid-containing cytoplasmic RNA labeled from 5 to 7 h after vaccinia virus infection formed intermolecular duplex structures characterized as double-stranded RNA by RNase resistance, density in Cs2SO4, base composition, chromatography on cellulose, and ability to inhibit reticulocyte cell-free protein synthesis. Both sucrose gradient sedimentation and electron microscopic analysis indicated that the double-stranded regions were several hundred to more than a thousand nucleotide base pairs long. The double-stranded RNA, after denaturation, hybridized to approximately 25% of the vaccinia virus genome, whereas total late RNA hybridized to 42%. The finding that the duplex RNA, after denaturation, hybridized to most HindIII restriction endonuclease fragments of vaccinia virus DNA indicated that symmetrical transcription is not confined to the terminal inverted repeat sequence or to one contiguous region of the genome. Although relatively little labeled, early, polyadenylic acid-containing RNA formed RNase-resistant hybrids upon self-annealing, the percentage increased upon addition of unlabeled late RNA, indicating that the latter contains "anti-early" sequences.
在痘苗病毒感染后5至7小时标记的含聚腺苷酸的细胞质RNA中，约15%形成了分子间双链结构，通过对核糖核酸酶的抗性、在硫酸铯中的密度、碱基组成、在纤维素上的色谱分析以及抑制网织红细胞无细胞蛋白质合成的能力，这些结构被表征为双链RNA。蔗糖梯度沉降和电子显微镜分析均表明，双链区域长度为几百至一千多个核苷酸碱基对。变性后的双链RNA与约25%的痘苗病毒基因组杂交，而晚期总RNA与42%的基因组杂交。变性后的双链RNA与痘苗病毒DNA的大多数HindIII限制性内切酶片段杂交，这一发现表明对称转录并不局限于末端反向重复序列或基因组的一个连续区域。尽管早期含聚腺苷酸的RNA标记相对较少，但自身退火时会形成对核糖核酸酶有抗性的杂交体，加入未标记的晚期RNA后，这一比例会增加，表明晚期RNA含有“抗早期”序列。
