痘苗病毒双链核糖核酸在体内和体外的合成机制
Mechanism of synthesis of vaccinia virus double-stranded ribonucleic acid in vivo and in vitro.
作者信息
Colby C, Jurale C, Kates J R
出版信息
J Virol. 1971 Jan;7(1):71-6. doi: 10.1128/JVI.7.1.71-76.1971.
Abstract
The synthesis of vaccinia virus double-stranded ribonucleic acid (RNA) in infected HeLa cells was sensitive to actinomycin D, suggesting that a deoxyribonucleic acid dependent reaction is involved. Some double-stranded RNA was made in the presence of cytosine arabinoside in infected cells. Double-stranded and complementary RNA were synthesized in vitro by using vaccinia cores. These two observations indicate that some of the double-stranded RNA is read from "early" genes. The double-stranded RNA synthesized in vitro had the same properties as that made in vivo. At least 70% of the double-stranded RNA made in vivo was in ribonuclease-resistant form prior to sodium dodecyl sulfate-phenol extraction. In addition, there was a complementary RNA in infected cells which could be converted to double-stranded RNA by annealing.
摘要
痘苗病毒双链核糖核酸（RNA）在受感染的HeLa细胞中的合成对放线菌素D敏感，这表明涉及一种依赖脱氧核糖核酸的反应。在受感染细胞中，一些双链RNA是在阿糖胞苷存在的情况下合成的。通过使用痘苗核心在体外合成了双链RNA和互补RNA。这两个观察结果表明，一些双链RNA是从“早期”基因转录而来的。体外合成的双链RNA与体内合成的具有相同的特性。在进行十二烷基硫酸钠-苯酚提取之前，体内合成的双链RNA中至少70%呈抗核糖核酸酶形式。此外，受感染细胞中存在一种互补RNA，它可以通过退火转化为双链RNA。
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