Spalding J W, Hook G E
Lipids. 1979 Jul;14(7):606-13. doi: 10.1007/BF02533444.
A soluble protein fraction (PLEP) prepared from rabbit lung can catalyze the exchange of phospholipids between subcellular organelles of the lung and between these subcellular organelles and synthetic liposomes. Phospholipid exchange between microsomes and synthetic liposomes and between mitochondria and synthetic liposomes was stimulated 8-fold and 2.5-fold, respectively, in the presence of the protein fraction. Lung exchange protein could also catalyzed phospholipid exchange between subcellular organelles of the liver and synthetic liposomes. Phospholipid transfer between microsomes and lamellar bodies of the lung was stimulated 2-fold by the exchange protein. Both radiolabeled phosphatidylcholine (PC) and phosphatidylinositol (PI) were transferred from 32P-labeled microsomes to lamellar bodies, but the exchange protein exhibited no transfer activity for phosphatidylglycerol (PG) and that for phosphatidylethanolamine (PE) was insignificant compared to the transfer activity for phosphatidylcholine and phosphatidylinositol. While the physiological role of the phospholipid exchange proteins in the lung is unknown, it is possible that they participate in the distribution of the newly synthesized phospholipids from the site of synthesis to lamellar bodies and other membrane compartments of cells.
从兔肺制备的可溶性蛋白组分（PLEP）可催化肺亚细胞器之间以及这些亚细胞器与合成脂质体之间的磷脂交换。在该蛋白组分存在的情况下，微粒体与合成脂质体之间以及线粒体与合成脂质体之间的磷脂交换分别被刺激了8倍和2.5倍。肺交换蛋白还可催化肝脏亚细胞器与合成脂质体之间的磷脂交换。交换蛋白使肺微粒体与板层小体之间的磷脂转移增加了2倍。放射性标记的磷脂酰胆碱（PC）和磷脂酰肌醇（PI）都从32P标记的微粒体转移到了板层小体，但交换蛋白对磷脂酰甘油（PG）没有转移活性，与对磷脂酰胆碱和磷脂酰肌醇的转移活性相比，对磷脂酰乙醇胺（PE）的转移活性微不足道。虽然肺中磷脂交换蛋白的生理作用尚不清楚，但它们有可能参与将新合成的磷脂从合成部位分布到板层小体和细胞的其他膜区室。
