从成年大鼠肺中分离的II型细胞中的磷脂转移活性。

Phospholipid-transfer activity in type II cells isolated from adult rat lung.

作者信息

Post M, Batenburg J J, Schuurmans E A, van Golde L M

出版信息

Biochim Biophys Acta. 1980 Nov 7;620(2):317-21. doi: 10.1016/0005-2760(80)90212-x.

Abstract

The soluble fraction from adult rat lung type II cells stimulated the transfer of various phospholipids between either liposomes or rat lung microsomes and rat lung mitochondria. Compared to whole lung, type II cells are highly enriched in transfer activity suggesting that phospholipid-transfer proteins play a role in the transport of surfactant phospholipids. Sephadex chromatography of pH 5.1 supernatant from type II cells yielded only one fraction catalysing the transfer of phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine, while chromatography of pH 5.1 supernatant from whole lung yielded in addition a specific phosphatidylcholine-transfer and a specific phosphatidylglycerol-transfer protein.

摘要

成年大鼠II型肺细胞的可溶性组分刺激了脂质体、大鼠肺微粒体和大鼠肺线粒体之间各种磷脂的转运。与全肺相比，II型细胞的转运活性高度富集，这表明磷脂转运蛋白在表面活性物质磷脂的转运中起作用。对II型细胞pH 5.1的上清液进行葡聚糖凝胶色谱分析，仅得到一个催化磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺转运的组分，而对全肺pH 5.1的上清液进行色谱分析，还得到了一种特异性磷脂酰胆碱转运蛋白和一种特异性磷脂酰甘油转运蛋白。