肺、分离的肺泡Ⅱ型细胞及肺泡Ⅱ型细胞源性腺瘤的胞质溶胶中的磷脂转移活性。
Phospholipid-transfer activities in cytosols from lung, isolated alveolar type II cells and alveolar type II cell-derived adenomas.
作者信息
Pool G L, Bubacz D G, Lumb R H, Mason R J
出版信息
Biochem J. 1983 Dec 1;215(3):637-42. doi: 10.1042/bj2150637.
We have examined phospholipid-transfer activities in cytosols from rat and mouse whole lung, isolated rat alveolar type II cells and alveolar type II cell-derived mouse pulmonary adenomas. We report an enrichment in phosphatidylcholine and phosphatidylglycerol (but not phosphatidylinositol) protein-catalysed transfer in the type II cell and adenoma cytosols compared with the whole-lung cytosols. The activities from these cytosols were resolved using column chromatofocusing, which clearly demonstrated the presence of a phosphatidylcholine-specific transfer protein in each of the four tissues. In addition, two proteins (rat) or three proteins (mouse) catalysing both phosphatidylcholine and phosphatidylglycerol transfer were resolved from whole lung, whereas in both the rat isolated alveolar type II cells and the mouse type II cell-derived adenomas one of these less specific proteins is not present.
我们检测了来自大鼠和小鼠全肺、分离的大鼠肺泡II型细胞以及肺泡II型细胞衍生的小鼠肺腺瘤的胞质溶胶中的磷脂转移活性。我们报告，与全肺胞质溶胶相比，II型细胞和腺瘤胞质溶胶中磷脂酰胆碱和磷脂酰甘油（而非磷脂酰肌醇）的蛋白催化转移有所富集。使用柱色谱聚焦法解析了这些胞质溶胶的活性，这清楚地证明了在这四种组织的每一种中都存在一种磷脂酰胆碱特异性转移蛋白。此外，从全肺中解析出了两种（大鼠）或三种（小鼠）催化磷脂酰胆碱和磷脂酰甘油转移的蛋白，而在大鼠分离的肺泡II型细胞和小鼠II型细胞衍生的腺瘤中，这些特异性较低的蛋白中的一种并不存在。
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