Kram D, Schneider E L, Senula G C, Nakanishi Y
Mutat Res. 1979 May;60(3):339-47. doi: 10.1016/0027-5107(79)90024-1.
Frequencies of sister-chromatid exchanges (SCE) were measured in vitro in mouse fibroblasts and in vivo in mouse bone-marrow cells. SCE levels in these cell systems were measured in response to varying concentrations of bromode-oxyuridine (BrdU) and mitomycin-C (MMC). Although BrdU was found to induce SCE in both cellular systems, baseline SCE levels were 2- to 3-fold higher in vitro than in vivo. SCE induction was found to be a linear function of MMC concentration in vivo and in vitro; however the slope of the vivo curve was 5-fold higher. The interaction of BrdU substituted DNA and MMC was examined by administering a fixed dose of MMC with increasing concentrations of BrdU. The induced SCE frequencies appeared to be additive. In addition to measuring drug-induced SCE, the BrdU differential staining technique allows concomitant measurement of the inhibition of cellular replication by the test drugs.
在体外对小鼠成纤维细胞以及在体内对小鼠骨髓细胞测量了姐妹染色单体交换（SCE）的频率。在这些细胞系统中，针对不同浓度的溴脱氧尿苷（BrdU）和丝裂霉素-C（MMC）测量了SCE水平。尽管发现BrdU在这两种细胞系统中均能诱导SCE，但体外的基线SCE水平比体内高2至3倍。发现在体内和体外，SCE诱导是MMC浓度的线性函数；然而，体内曲线的斜率高5倍。通过给予固定剂量的MMC并增加BrdU的浓度，研究了BrdU替代DNA与MMC的相互作用。诱导的SCE频率似乎是相加的。除了测量药物诱导的SCE外，BrdU差异染色技术还允许同时测量受试药物对细胞复制的抑制作用。
