Willett J D, Downey W L
Biochem J. 1974 Feb;138(2):233-7. doi: 10.1042/bj1380233.
The fate of the known sterol precursor squalene 2,3-oxide was investigated in the free-living nematode Panagrellus redivivus. The nematodes were cultured axenically in the presence of [4-(3)H]squalene 2,3-oxide. Radioactivity was found in the total lipids of the isolated nematodes. Essentially all of the radioactivity encountered in the total lipids was found in the non-saponifiable fraction. The components present in the non-saponifiable fraction were separated and isolated by t.l.c. Three labelled components were identified by a combination of t.l.c., g.l.c. and mass spectroscopy. It is established that P. redivivus has the capacity for biosynthesis of lanosterol. No labelled C(27) sterols could be detected.
在自由生活的线虫秀丽隐杆线虫中研究了已知的甾醇前体角鲨烯2,3-氧化物的命运。线虫在[4-(3)H]角鲨烯2,3-氧化物存在下进行无菌培养。在分离出的线虫的总脂质中发现了放射性。总脂质中基本上所有的放射性都存在于不皂化部分。通过薄层层析将不皂化部分中的成分分离并分离出来。通过薄层层析、气相色谱和质谱联用鉴定了三种标记成分。已确定秀丽隐杆线虫具有生物合成羊毛甾醇的能力。未检测到标记的C(27)甾醇。
