通过电子显微镜观察，用铂 - 嘧啶复合物使致瘤细胞中与细胞表面相关的核酸可见。 - Suppr

Aggarwal S K, Wagner R W, McAllister P K, Rosenberg B

Proc Natl Acad Sci U S A. 1975 Mar;72(3):928-32. doi: 10.1073/pnas.72.3.928.

Platinum-pyrimidine complexes have been used as the sole electron-dense stains for electron microscopy on 18 types of mammalian cells. These recently discovered complexes react avidly with nucleic acids in vitro, are antitumor agents, and are highly soluble in water. In cells, they are selective for regions rich in nucleic acid and make visible the cellular chromatin, nucleolus, and ribosomes. In addition, cells that are tumorigenic exhibit electron-dense patches at the plasma membrane; normal cells do not. The results of treatment of ascites sarcoma-180 cells with concanavalin A, nucleases, glycosidases, or a protease suggest that the electron-dense patches are DNA, with neuroaminic acid involved in the surface binding.

铂-嘧啶复合物已被用作18种哺乳动物细胞电子显微镜检查的唯一电子致密染色剂。这些最近发现的复合物在体外能与核酸发生强烈反应，是抗肿瘤药物，且在水中高度可溶。在细胞中，它们对富含核酸的区域具有选择性，能使细胞染色质、核仁和核糖体可见。此外，具有致瘤性的细胞在质膜处呈现电子致密斑块；正常细胞则没有。用伴刀豆球蛋白A、核酸酶、糖苷酶或蛋白酶处理腹水肉瘤-180细胞的结果表明，这些电子致密斑块是DNA，且神经氨酸参与表面结合。