Wahl S M, Arend W P, Ross R
Am J Pathol. 1974 Apr;75(1):73-89.
The role of the complement system in nonspecific inflammation was investigated by depleting guinea pigs of serum complement with cobra venom factor (CoF). The progress of wound healing was compared in decomplemented and control animals which received buffer and no CoF. When wound tissue sections were assessed at 12 hours after wounding, no morphologic differences were observed between experimental and control wounds. Quantitation of 24-hour wound exudates by a point volumetric method revealed a 50% reduction in infiltrating polymorphonuclear neutrophilic leukocytes (PMN) in the absence of complement. A smaller decrease in mononuclear leukocytes (MNL) was seen. Red cells occupied four times as much space in the experimental wounds at 24 hours. In 48-hour wounds, PMN were still 50% of controls, but were near control levels at 72 hours. Despite the decreased influx of PMN, wound debridement and subsequent fibrogenesis proceeded as in the controls. No differences were seen in fibroblast proliferation, connective tissue formation or capillary regeneration. These results suggest that the complement system is not a primary mediator of inflammation following a nonimmunologic stimulus.
通过用眼镜蛇毒因子（CoF）去除豚鼠血清补体，研究补体系统在非特异性炎症中的作用。比较了接受缓冲液且未接受CoF的补体缺乏动物和对照动物的伤口愈合进程。在受伤后12小时评估伤口组织切片时，实验性伤口和对照伤口之间未观察到形态学差异。通过点体积法对24小时伤口渗出液进行定量分析发现，在缺乏补体的情况下，浸润的多形核中性粒细胞（PMN）减少了50%。单核细胞（MNL）的减少幅度较小。在24小时时，红细胞在实验性伤口中所占空间是对照伤口的四倍。在48小时的伤口中，PMN仍为对照的50%，但在72小时时接近对照水平。尽管PMN的流入减少，但伤口清创及随后的纤维形成过程与对照相同。在成纤维细胞增殖、结缔组织形成或毛细血管再生方面未观察到差异。这些结果表明，补体系统不是非免疫刺激后炎症的主要介质。
