Ross R, Odland G
J Cell Biol. 1968 Oct;39(1):152-68. doi: 10.1083/jcb.39.1.152.
Connective tissue repair was studied in a series of skin wounds in young adult males. The tissues were examined at 3, 12, and 24 hr, and at 2, 3, 5, 7, 14, and 21 days after wounding. The neutrophilic leukocytes contain within membrane-bounded vacuoles some fibrin and serum protein from the wound; however, most of the granulocytes lyse and release their cytoplasmic contents into the extracellular space. The mononuclear cells undergo a series of morphologic alterations during which they develop a modest amount of relatively poorly developed rough endoplasmic reticulum and an extensive system of smooth-surfaced membranes prior to active phagocytosis. They could be clearly distinguished from immature fibroblasts by the differences in the development of their organelles, particularly the rough endoplasmic reticulum. The perivascular connective tissue adjacent to the wound contains cells which appear like poorly developed or immature fibroblasts. The development of these cells into mature fibroblasts can be followed during the different stages of wound repair. Intimate contact was observed between basal cells of the regenerated epidermis and monocytes in the wound below: cytoplasmic projections of the basal cells extended beneath the basement lamina to the surface of the monocytes. Such contacts were seen only on the 4th-7th day after wounding. Their possible significance is discussed.
在一系列年轻成年男性的皮肤伤口中研究了结缔组织修复情况。在受伤后3小时、12小时和24小时，以及2天、3天、5天、7天、14天和21天对组织进行检查。中性粒细胞在膜结合的空泡内含有一些来自伤口的纤维蛋白和血清蛋白；然而，大多数粒细胞会溶解并将其细胞质内容物释放到细胞外空间。单核细胞经历一系列形态学改变，在此期间，它们在活跃吞噬之前会发育出少量发育相对较差的粗面内质网和广泛的光滑表面膜系统。通过细胞器发育的差异，尤其是粗面内质网的差异，可以将它们与未成熟的成纤维细胞清楚地区分开来。伤口附近的血管周围结缔组织含有看起来像发育不良或未成熟成纤维细胞的细胞。在伤口修复的不同阶段可以追踪这些细胞向成熟成纤维细胞的发育过程。在下方伤口的再生表皮基底细胞和单核细胞之间观察到紧密接触：基底细胞的细胞质突起延伸到基膜下方至单核细胞表面。这种接触仅在受伤后第4 - 7天可见。讨论了它们可能的意义。
