Trigg P H, Belin R, Haberkorn S, Long W J, Nixon H H, Plaschkes J, Spitz L, Willital G H
J Clin Pathol. 1974 Mar;27(3):207-13. doi: 10.1136/jcp.27.3.207.
Cryostat sections from 160 rectal suction biopsies were stained for cholinesterases by the method of Karnovsky and Roots (1964) in an attempt to facilitate the diagnosis of Hirschsprung's disease. The method proved at least as reliable as experienced assessment of paraffin haematoxylin-eosin sections, and appeared to offer the advantages of reduced scanning fatigue and superior demonstration of the increased cholinesterase-positive nerves in Hirschprung's disease. Contrary to the findings of Meier-Ruge (1971) it was not possible to base a diagnosis on mucosal cholinesterase activity.
为便于诊断先天性巨结肠，对160份直肠吸引活检的低温恒温器切片采用卡诺夫斯基和鲁茨（1964年）的方法进行胆碱酯酶染色。该方法证明至少与经验丰富的石蜡苏木精-伊红切片评估一样可靠，并且似乎具有减少扫描疲劳以及能更好地显示先天性巨结肠中胆碱酯酶阳性神经增多的优点。与迈尔-鲁格（1971年）的研究结果相反，不可能根据黏膜胆碱酯酶活性进行诊断。
