Makino I, Shinozaki K, Nakagawa S, Mashimo K
J Lipid Res. 1974 Mar;15(2):132-8.
Amberlite XAD-2 was used to extract bile acids from urine or diluted serum of patients with hepatobiliary diseases. Columns containing Sephadex LH-20 were then used to separate the sulfated and nonsulfated bile acids. Thin-layer chromatography of the sulfated bile acid fraction obtained from urine revealed several spots with R(F) values different from those of the taurine or glycine conjugates. According to thin-layer chromatographic mobilities, gas-liquid chromatographic analyses, infrared spectra, and elementary analysis of the sulfated material, one of these sulfated bile acids was identified as glycochenodeoxycholic acid monosulfate, and the others were presumed to be taurochenodeoxycholic acid sulfate and glycocholic acid sulfate. A large amount of bile acid sulfate was found in urine of patients with hepatobiliary diseases. They accounted for 35.5-93.3% of total urinary bile acids and consisted of both di- and trihydroxycholanoic acids, with chenodeoxycholic acid as the major acid. Sulfated bile acids were also found in serum, and accounted for 1.8-21.2% of the total bile acids. Only dihydroxycholanoic acids (mainly chenodeoxycholic) were identified.
采用Amberlite XAD - 2从肝胆疾病患者的尿液或稀释血清中提取胆汁酸。然后使用装有Sephadex LH - 20的柱分离硫酸化和非硫酸化胆汁酸。对从尿液中获得的硫酸化胆汁酸部分进行薄层层析，结果显示有几个斑点，其R(F)值与牛磺酸或甘氨酸缀合物的R(F)值不同。根据硫酸化物质的薄层层析迁移率、气液色谱分析、红外光谱和元素分析，其中一种硫酸化胆汁酸被鉴定为甘氨鹅去氧胆酸单硫酸盐，其他的推测为牛磺鹅去氧胆酸硫酸盐和甘胆酸硫酸盐。在肝胆疾病患者的尿液中发现了大量的硫酸化胆汁酸。它们占总尿胆汁酸的35.5 - 93.3%，由二羟基和三羟基胆烷酸组成，其中鹅去氧胆酸是主要的酸。在血清中也发现了硫酸化胆汁酸，占总胆汁酸的1.8 - 21.2%。仅鉴定出二羟基胆烷酸（主要是鹅去氧胆酸）。
