Auger J G, Shalla T A, Kado C I
Science. 1974 Jun 28;184(4144):1375-7. doi: 10.1126/science.184.4144.1375.
A Gram-positive, rod-shaped bacterium was isolated and grown in pure culture on artificial mediums from the leafhopper Draeculacephala minerva Ball which had fed on plants infected with Pierce's disease. The bacterial culture was injected into uncontaminated leafhoppers which were than allowed to feed on healthy grapevines. After exposure to these leafhoppers, typical symptoms of Pierce's disease developed; however, no symptoms developed on plants exposed to leafhoppers injected with sterile culture medium. The same organism was reisolated from the experimentally inoculated plants. Electron microscopic examination of these infected plants revealed bacterial cells localized in the xylem tissues. No such cells were seen in healthy or control plants.
从以感染皮尔斯病的植物为食的小叶蝉（Draeculacephala minerva Ball）中分离出一种革兰氏阳性杆状细菌，并在人工培养基上进行纯培养。将该细菌培养物注射到未受污染的小叶蝉体内，然后让它们取食健康的葡萄藤。接触这些小叶蝉后，出现了皮尔斯病的典型症状；然而，接触注射了无菌培养基的小叶蝉的植物没有出现症状。从实验接种的植物中重新分离出了相同的生物体。对这些受感染植物进行电子显微镜检查发现，细菌细胞定位于木质部组织中。在健康或对照植物中未观察到此类细胞。
