Mollenhauer H H, Hopkins D L
J Bacteriol. 1974 Aug;119(2):612-8. doi: 10.1128/jb.119.2.612-618.1974.
The rod-shaped rickettsia-like bacteria of Pierce's disease measure about 0.25 to 0.50 mum in diameter and 1.0 to 4.0 mum long. The bacteria have a cell wall consisting of a trilaminar outer membrane and two intermediate low-density layers separated by a dense intermediate layer. A trilaminar cytoplasmic membrane is also present, resulting in a total wall complex thickness of 25 to 40 nm. A periodic infolding of the outer membrane and intermediate layers of the wall give the wall surface a ridged apperance. The ridges appear to go around the long axis of the cell, possibly in the form of spirals. Ribosomes and nuclear regions with easily visible deoxyribonucleic acid strands and clumps are distributed throughout the cytoplasm. Binary fission, during which the cell wall and cytoplasmic membrane folded inward to partition the cell, was observed. In the xylem of infected grapes, the bacteria are either distributed evenly throughout the lumen of the xylem vessel or appressed along the inner surface of the vessel walls in an electron-lucent matrix.
皮尔氏病中杆状的类立克次氏体细菌直径约为0.25至0.50微米，长度为1.0至4.0微米。这些细菌具有细胞壁，细胞壁由三层外膜和两层中间低密度层组成，中间由一层致密的中间层隔开。还存在一层三层的细胞质膜，导致整个壁复合体厚度为25至40纳米。外膜和壁的中间层的周期性内褶使壁表面呈现出有脊的外观。这些脊似乎围绕细胞的长轴，可能呈螺旋形式。核糖体和带有易于观察到的脱氧核糖核酸链和团块的核区域分布在整个细胞质中。观察到了二分裂过程，在此过程中细胞壁和细胞质膜向内折叠以分隔细胞。在受感染葡萄的木质部中，细菌要么均匀分布在木质部导管的管腔中，要么在电子透明基质中沿着导管壁的内表面紧贴分布。
