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用对流免疫电泳法检测棘球蚴病患者沉淀抗体(作者译)

[Demonstration of Precipitating antibodies in patients with echinococcosis using a counter immunoelectrophoresis (author's transl)].

作者信息

Todorov T, Jeleva R

出版信息

Tropenmed Parasitol. 1979 Jun;30(2):182-8.

PMID:483382
Abstract

A counter immunoelectrophoresis was used for the demonstration of antibodies to echinococci. The antigen was prepared from hydatid fluid obtained from liver cysts of sheep. After concentration the antigenic material contained 1800 to 2900 micrograms of nitrogen per milliliter fluid. One or more precipitating lines were regarded as positive. Serum samples of 184 patients with surgically proven echinococcosis gave positive results in 80,4% of the cases. Among them, patients with hydatid disease of the liver were positive in 83,2% of the cases, and patients with hydatid disease of the lung were positive in 78,6 of the cases. The arithmetric mean of the number of lines was 2,4. Sera of 1182 patients with other diseases (among others diseases of the gastro-intestinal and respiratory tract, allergies, neuro-psychiatric, gynecologic and parasitic diseases) reached in 4,6% of the cases with the formation of precipitating lines. The described test is simple to perform, it can be read after a short period of time and it can be used as a screening test for mass case finding.

摘要

采用对流免疫电泳法检测棘球蚴抗体。抗原取自绵羊肝囊肿的囊液。浓缩后,每毫升抗原物质含1800至2900微克氮。出现一条或多条沉淀线视为阳性。184例经手术证实患有棘球蚴病患者的血清样本中,80.4%呈阳性结果。其中,肝包虫病患者阳性率为83.2%,肺包虫病患者阳性率为78.6%。沉淀线数量的算术平均值为2.4。1182例患有其他疾病(包括胃肠道和呼吸道疾病、过敏、神经精神疾病、妇科疾病和寄生虫病等)患者的血清中,4.6%出现沉淀线。所述检测方法操作简单,短时间内即可读取结果,可作为大规模病例筛查的检测方法。

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