Mansueto S, Migneco G, Tripi S, Picone D, Miceli D
Boll Ist Sieroter Milan. 1979 Jul 31;58(3):260-5.
A very simplified method of crossed over electrophoresis (CIEP) was employed with a lyophilized commercially produced antigen (previously submitted to several freezing and thawing) and cellulose acetate membrane, for the diagnosis of human hydatidosis. Results are as follows: active hydatidosis (surgically confirmed): number of sera tested 35; positive 32 (91%). Sera of patients with parasitic and non parasitic illnesses (especially malignancies of liver and lung) did not show any precipitin lines. The pattern of immunoprecipitation is characteristically in the form of an "upper lip" or a thick and undulating "streak". The very thin arcs of precipitation which are occurring in some control sera, are to be considered negative. These results indicate that with regard to the rule of the "three S" (specificity, sensitivity and simplicity) the method appears sufficiently satisfactory.
采用一种非常简化的交叉免疫电泳（CIEP）方法，使用冻干的商业生产抗原（先前经过多次冻融）和醋酸纤维素膜来诊断人类包虫病。结果如下：活动性包虫病（手术确诊）：检测血清数量35份；阳性32份（91%）。患有寄生虫病和非寄生虫病（尤其是肝和肺恶性肿瘤）患者的血清未显示任何沉淀线。免疫沉淀模式典型地呈“上唇”形式或厚且起伏的“条纹”形式。在一些对照血清中出现的非常细的沉淀弧应视为阴性。这些结果表明，就“三S”原则（特异性、敏感性和简易性）而言，该方法似乎足够令人满意。
