在泛影葡胺溶液中对染色质进行等密度离心。
Isopycnic centrifugation of chromatin in renografin solutions.
作者信息
Chan R T, Scheffler I E
出版信息
J Cell Biol. 1974 Jun;61(3):780-8. doi: 10.1083/jcb.61.3.780.
Abstract
Solutions of Renografin (30-60%) can be centrifuged to form density gradients in the range from 1.0 g/cm(3) to 1.4 g/cm(3) or, alternatively, preformed gradients can be made which under appropriate conditions of centrifugation have an indefinite stability. Such solutions have a low viscosity and a relatively low ionic strength. The density of DNA in such solutions is surprisingly low ( approximately 1.14 g/cm(3)). Crude chromatin can be sedimented to an equilibrium position in such gradients, corresponding to a density of 1.2(4) g/cm(3), or slightly lower, depending on the method of preparation. The complex is shown to contain DNA, RNA, protein, and possibly some lipoprotein. Most of the RNA can be removed with RNase without any significant effect on the density of the chromatin.
摘要
泛影葡胺溶液（30%-60%）可经离心形成密度范围为1.0克/立方厘米至1.4克/立方厘米的密度梯度，或者，也可制备预制梯度，在适当的离心条件下，其具有无限稳定性。此类溶液粘度低，离子强度相对较低。DNA在此类溶液中的密度出奇地低（约1.14克/立方厘米）。粗染色质可在此类梯度中沉降至平衡位置，对应密度为1.2(4)克/立方厘米，或略低，具体取决于制备方法。该复合物显示含有DNA、RNA、蛋白质，可能还含有一些脂蛋白。大部分RNA可用核糖核酸酶去除，而对染色质的密度无任何显著影响。
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The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action.孤立染色质的生物学：在试管中具有生物活性的染色体为研究基因作用提供了一个有力的工具。
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