• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种降解洗涤剂的微生物中伯烷基硫酸酯酶和芳基硫酸酯酶的作用机制。

The mechanism of action of primary alkylsulphohydrolase and arylsulphohydrolase from a detergent-degrading micro-organism.

作者信息

Cloves J M, Dodgson K S, Games D E, Shaw D J, White G F

出版信息

Biochem J. 1977 Dec 1;167(3):843-6. doi: 10.1042/bj1670843.

DOI:10.1042/bj1670843
PMID:603638
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183735/
Abstract

Previous studies have shown that secondary alkylsulphohydrolases from certain detergent-degrading micro-organisms are unusual esterases in that they catalyse fission of the C-O bond of the alkyl sulphate ester linkage. The position of bond fission catalysed by a primary alkylsulphatase and an arylsulphohydrolase present in Pseudomonas C12B has now been investigated. The primary alkylsulphatase behaved like the secondary alkylsulphohydrolases in cleaving the C-O bond of potassium heptan-1-yl sulphate. In contrast, the arylsulphohydrolase, in common with other similar enzymes previously studied, catalysed the fission of the O-S bond of potassium p-nitrophenyl sulphate.

摘要

以往的研究表明，某些降解洗涤剂的微生物中的仲烷基硫酸酯酶是特殊的酯酶，因为它们催化烷基硫酸酯键的C-O键断裂。现在已经研究了假单胞菌C12B中存在的伯烷基硫酸酯酶和芳基硫酸酯酶催化的键断裂位置。伯烷基硫酸酯酶在裂解庚烷-1-基硫酸钾的C-O键时，表现得与仲烷基硫酸酯酶相似。相反，芳基硫酸酯酶与先前研究的其他类似酶一样，催化对硝基苯硫酸钾的O-S键断裂。

相似文献

1
The mechanism of action of primary alkylsulphohydrolase and arylsulphohydrolase from a detergent-degrading micro-organism.一种降解洗涤剂的微生物中伯烷基硫酸酯酶和芳基硫酸酯酶的作用机制。
Biochem J. 1977 Dec 1;167(3):843-6. doi: 10.1042/bj1670843.
2
Purification and properties of the S1 secondary alkylsulphohydrolase of the detergent-degrading micro-organism, Pseudomonas C12B.降解洗涤剂的微生物假单胞菌C12B的S1仲烷基磺基水解酶的纯化及性质
Biochem J. 1978 Mar 1;169(3):659-67. doi: 10.1042/bj1690659.
3
Preparation and characterization of substrates suitable for the study of stereospecific secondary alkylsulphohydrolases of detergent-degrading micro-organisms.适用于研究洗涤剂降解微生物立体特异性仲烷基硫酸水解酶的底物的制备与表征
Biochem J. 1977 Dec 1;167(3):717-22. doi: 10.1042/bj1670717.
4
A novel mechanism of enzymic ester hydrolysis. Inversion of configuration and carbon-oxygen bond cleavage by secondary alkylsulphohydrolases from detergent-degrading micro-organisms.一种酶促酯水解的新机制。来自洗涤剂降解微生物的仲烷基硫水解酶导致构型翻转和碳 - 氧键断裂。
Biochem J. 1977 Sep 1;165(3):575-80. doi: 10.1042/bj1650575.
5
Primary alkylsulphatase activities of the detergent-degrading bacterium Pseudomonas C12B. Purification and properties of the P1 enzyme.洗涤剂降解菌假单胞菌C12B的初级烷基硫酸酯酶活性。P1酶的纯化及性质
Biochem J. 1986 Jun 1;236(2):401-8. doi: 10.1042/bj2360401.
6
Substrate specificity and other properties of the inducible S3 secondary alkylsulphohydrolase purified from the detergent-degrading bacterium Pseudomonas C12B.从降解洗涤剂的假单胞菌C12B中纯化得到的可诱导S3仲烷基硫水解酶的底物特异性及其他特性。
Biochem J. 1980 Apr 1;187(1):181-90. doi: 10.1042/bj1870181.
7
Chemically defined inducers of alkylsulphatases present in Pseudomonas C12B.假单胞菌C12B中存在的烷基硫酸酯酶的化学定义诱导剂。
Biochem J. 1974 Jan;138(1):53-62. doi: 10.1042/bj1380053.
8
Specificity of P2 primary alkylsulphohydrolase induction in the detergent-degrading bacterium Pseudomonas C12B. Effects of alkanesulphonates, alkyl sulphates and other related compounds.洗涤剂降解菌假单胞菌C12B中P2伯烷基硫醇水解酶诱导的特异性。链烷磺酸盐、烷基硫酸盐及其他相关化合物的影响。
Biochem J. 1980 Jan 1;185(1):13-21. doi: 10.1042/bj1850013.
9
Induction of primary alkysulphatases and metabolism of sodium hexan-1-yl sulphate by Pseudomonas C12B.假单胞菌C12B对初级烷基硫酸酯酶的诱导及己烷-1-基硫酸钠的代谢
Biochem J. 1974 Jan;138(1):63-9. doi: 10.1042/bj1380063.
10
An improved procedure for the preparation of alkyl sulphate esters suitable for the study of secondary alkylsulphohydrolase enzymes.一种改进的制备适用于仲烷基硫酸酯酶研究的烷基硫酸酯的方法。
Biochem J. 1980 Apr 1;187(1):191-6. doi: 10.1042/bj1870191.

引用本文的文献

1
Microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities.微生物烷基-和芳基-硫酸酯酶：机制、发生、筛选和立体选择性。
Appl Microbiol Biotechnol. 2014 Feb;98(4):1485-96. doi: 10.1007/s00253-013-5438-0. Epub 2013 Dec 19.
2
Initiation of Activation of a Preemergent Herbicide by a Novel Alkylsulfatase of Pseudomonas putida FLA.新型恶臭假单胞菌 FLA 烷基磺酸盐酶对芽前除草剂的激活作用。
Appl Environ Microbiol. 1983 Nov;46(5):988-94. doi: 10.1128/aem.46.5.988-994.1983.
3
Novel alkylsulfatases required for biodegradation of the branched primary alkyl sulfate surfactant 2-butyloctyl sulfate.支链伯烷基硫酸盐表面活性剂2-丁基辛基硫酸盐生物降解所需的新型烷基硫酸酯酶。
Appl Environ Microbiol. 2002 Jan;68(1):31-6. doi: 10.1128/AEM.68.1.31-36.2002.
4
Purification and characterization of the short-chain alkylsulphatase of coryneform B1a.棒状杆菌B1a短链烷基硫酸酯酶的纯化与特性分析
Biochem J. 1994 Dec 15;304 ( Pt 3)(Pt 3):937-43. doi: 10.1042/bj3040937.
5
Substrate specificity and other properties of the inducible S3 secondary alkylsulphohydrolase purified from the detergent-degrading bacterium Pseudomonas C12B.从降解洗涤剂的假单胞菌C12B中纯化得到的可诱导S3仲烷基硫水解酶的底物特异性及其他特性。
Biochem J. 1980 Apr 1;187(1):181-90. doi: 10.1042/bj1870181.
6
Stimulation of bacterial arylsulfatase activity by arylamines: evidence for substrate activation.芳胺对细菌芳基硫酸酯酶活性的刺激作用：底物激活的证据。
J Bacteriol. 1981 Jul;147(1):69-74. doi: 10.1128/jb.147.1.69-74.1981.
7
Purification and properties of the P2 primary alkylsulphohydrolase of the detergent-degrading bacterium pseudomonas C12B.洗涤剂降解细菌假单胞菌C12B的P2伯烷基磺基水解酶的纯化及性质
Biochem J. 1980 Jan 1;185(1):23-31. doi: 10.1042/bj1850023.
8
Primary alkylsulphatase activities of the detergent-degrading bacterium Pseudomonas C12B. Purification and properties of the P1 enzyme.洗涤剂降解菌假单胞菌C12B的初级烷基硫酸酯酶活性。P1酶的纯化及性质
Biochem J. 1986 Jun 1;236(2):401-8. doi: 10.1042/bj2360401.
9
Oxygen-dependent desulphation of monomethyl sulphate by Agrobacterium sp. M3C.农杆菌属M3C对单甲基硫酸盐的需氧脱硫作用
Biodegradation. 1990;1(4):229-41. doi: 10.1007/BF00119760.

本文引用的文献

1
Studies on sulphatases. 25. The determination of BaSO(3)O by infrared spectroscopy.硫酸酯酶研究。25. 用红外光谱法测定碱式硫酸钡。
Biochem J. 1959 Nov;73(3):442-7. doi: 10.1042/bj0730442.
2
Studies on sulphatases. 20. Enzymic cleavage of aryl hydrogen sulphates in the presence of H218O.硫酸酯酶研究。20. 在H218O存在下芳基硫酸氢酯的酶促裂解。
Biochem J. 1958 May;69(1):155-9. doi: 10.1042/bj0690155.
3
Assay of sulfatases.硫酸酯酶测定
Methods Biochem Anal. 1957;4:211-55. doi: 10.1002/9780470110201.ch6.
4
The enzymic degradation of L-serine O-sulphate by a specific system from pig liver. Studies on the mechanism of the reaction.猪肝特定系统对L-丝氨酸O-硫酸盐的酶促降解。反应机制研究。
Biochem J. 1972 Jan;126(1):187-91. doi: 10.1042/bj1260187.
5
Chemically defined inducers of alkylsulphatases present in Pseudomonas C12B.假单胞菌C12B中存在的烷基硫酸酯酶的化学定义诱导剂。
Biochem J. 1974 Jan;138(1):53-62. doi: 10.1042/bj1380053.
6
Induction in a Pseudomonas species of sulphatases active on short chain alkylsulphates.
Microbios. 1972 Mar-Apr;5(18):87-100.
7
The regulation of arylsulphatase formation in Pseudomonas C 12 B.假单胞菌C 12 B中芳基硫酸酯酶形成的调控
Microbios. 1972 Sep-Oct;6(22):147-56.
8
Methods for visualization of enzymes in polyacrylamide gels.聚丙烯酰胺凝胶中酶可视化的方法。
Appl Microbiol. 1974 Jan;27(1):154-8. doi: 10.1128/am.27.1.154-158.1974.
9
Secondary alkylsulphatases in a strain of Comamonas terrigena.一株陶氏丛毛单胞菌中的仲烷基硫酸酯酶
Biochem J. 1975 Aug;149(2):477-80. doi: 10.1042/bj1490477.
10
A novel mechanism of enzymic ester hydrolysis. Inversion of configuration and carbon-oxygen bond cleavage by secondary alkylsulphohydrolases from detergent-degrading micro-organisms.一种酶促酯水解的新机制。来自洗涤剂降解微生物的仲烷基硫水解酶导致构型翻转和碳 - 氧键断裂。
Biochem J. 1977 Sep 1;165(3):575-80. doi: 10.1042/bj1650575.