菠菜叶片过氧化物酶体和线粒体对甲酸的氧化作用。
Oxidation of formate by peroxisomes and mitochondria from spinach leaves.
作者信息
Halliwell B
出版信息
Biochem J. 1974 Jan;138(1):77-85. doi: 10.1042/bj1380077.
Abstract
- Spinach (Spinacia oleracea L.) leaf extracts catalyse the oxidation of formate to CO(2). 2. Two enzymic systems are responsible for this oxidation, the peroxidatic action of catalase (EC 1.11.1.6) and NAD-dependent formate dehydrogenase (EC 1.2.1.2). 3. Formate dehydrogenase is mainly, if not exclusively, located in the mitochondria. This enzyme has a pH optimum of 6-6.5 and a K(m) for formate of 1.7mm in the presence of 1 mm-NAD(+). 4. Peroxidatic action of catalase is presumed to take place in peroxisomes, since these seem to be the subcellular site of catalase. Formate oxidation at pH5 by chloroplast and mitochondrial fractions is due to their ability to generate H(2)O(2) and the presence of contaminating catalase. 5. During photorespiration, peroxidatic oxidation of formate by catalase can occur over a wide range of pH values, but the rate of this reaction is probably controlled by the concentration of formate present, to an extent dependent on the pH.
摘要
- 菠菜（Spinacia oleracea L.）叶提取物可催化甲酸盐氧化为二氧化碳。2. 两种酶系统负责这种氧化反应，即过氧化氢酶（EC 1.11.1.6）的过氧化物酶作用和NAD依赖的甲酸盐脱氢酶（EC 1.2.1.2）。3. 甲酸盐脱氢酶主要（如果不是唯一的话）位于线粒体中。在1 mM - NAD⁺存在的情况下，该酶的最适pH为6 - 6.5，甲酸盐的米氏常数（K(m)）为1.7 mM。4. 过氧化氢酶的过氧化物酶作用被认为发生在过氧化物酶体中，因为过氧化物酶体似乎是过氧化氢酶的亚细胞定位。叶绿体和线粒体组分在pH5时对甲酸盐的氧化是由于它们产生过氧化氢的能力以及存在污染的过氧化氢酶。5. 在光呼吸过程中，过氧化氢酶对甲酸盐的过氧化物酶氧化可在很宽的pH值范围内发生，但该反应的速率可能受甲酸盐浓度控制，在一定程度上取决于pH值。
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