Paulson D F, Bonar R A, Sharief Y, Vergara J R, Reich C, Shah K V
Cancer Chemother Rep. 1975 Jan-Feb;59(1):51-5.
SV-40-transformed hamster prostatic tissue has been previously evaluated as a model for human prostatic carcinoma. Because the original cell line was lost, Syrian golden hamster prostatic tissue has been established in explant culture and infected with a 10-6-cell tissue culture infectious dose (50 percent effective) of SV40. After in vitro transformation, the cells were produced in quantity and 60 times 10-6 cells were injected into adult male Syrian golden hamsters 24 hours after 400 rads of whole-body radiation. After 60-90 days, a small palpable tumor developed. These tumors could be serially transplanted in adult male animals without immunosuppression. The tumor cells were established in tissue culture and the cells were returned to adult animals without immunosuppression where they rapidly produced fast-growing tumors. The solid tumors were composed of sheets of pleomorphic polygonal cells with large nuclei and many nucleoli; they resembled undifferentiated human prostatic carcinoma. In vitro, the cultures contained small, rapidly growing cells with a population doubling time of about 1.3 days. The cells carried the SV 40-specific antigen. The modal chromosome number was 66-68 with a distribution of 47-120. Cells exposed to 2-bromo-5'-deoxyuridine in culture did not release particles with RNA-dependent DNA polymerase activity. Endocrine sensitivity in vivo and in vitro is undertermined to date.
SV - 40转化的仓鼠前列腺组织先前已被评估为人类前列腺癌的模型。由于原始细胞系丢失，叙利亚金黄仓鼠前列腺组织已通过外植体培养建立，并感染了10^-6细胞组织培养感染剂量（50%有效）的SV40。体外转化后，大量产生细胞，并在400拉德全身辐射24小时后，将60×10^-6个细胞注射到成年雄性叙利亚金黄仓鼠体内。60 - 90天后，出现一个可触及的小肿瘤。这些肿瘤可以在成年雄性动物中连续传代移植而无需免疫抑制。肿瘤细胞在组织培养中建立，然后在不进行免疫抑制的情况下将细胞回输到成年动物体内，它们迅速产生生长迅速的肿瘤。实体瘤由成片的多形性多边形细胞组成，细胞核大，有许多核仁；它们类似于未分化的人类前列腺癌。在体外，培养物中含有小的、生长迅速的细胞，群体倍增时间约为1.3天。细胞携带SV 40特异性抗原。众数染色体数为66 - 68，分布范围为47 - 120。培养中暴露于2 - 溴 - 5'-脱氧尿苷的细胞未释放具有RNA依赖性DNA聚合酶活性的颗粒。迄今为止，体内和体外的内分泌敏感性尚未确定。
