细菌脂肪酸分析快速方法的比较
Comparison of rapid methods for analysis of bacterial fatty acids.
作者信息
Moss C W, Lambert M A, Merwin W H
出版信息
Appl Microbiol. 1974 Jul;28(1):80-5. doi: 10.1128/am.28.1.80-85.1974.
Abstract
When rapid gas-liquid chromatography methods for determination of bacterial fatty acids were compared, results showed that saponification was required for total fatty acid analysis. Transesterification with boron-trihalide reagents (BF(3)-CH(3)OH, BCl(3)-CH(3)OH) caused extensive degradation of cyclopropane acids and was less effective than saponification in releasing cellular hydroxy fatty acids. Digestion of cells with tetramethylammonium hydroxide was unsatisfactory because of extraneous gas-liquid chromatography peaks and because of lower recovery of branched-chain and hydroxy fatty acids. A simple, rapid saponification procedure which can be used for total cellular fatty acid analysis of freshly grown cells is described.
摘要
在比较用于测定细菌脂肪酸的快速气液色谱法时，结果表明总脂肪酸分析需要皂化。用三卤化硼试剂（BF(3)-CH(3)OH、BCl(3)-CH(3)OH）进行酯交换会导致环丙烷酸大量降解，并且在释放细胞羟基脂肪酸方面不如皂化有效。用氢氧化四甲铵消化细胞并不理想，因为会出现额外的气液色谱峰，并且支链脂肪酸和羟基脂肪酸的回收率较低。本文描述了一种简单、快速的皂化程序，可用于对新鲜培养细胞进行总细胞脂肪酸分析。
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本文引用的文献
1
CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY.通过化学成分分析对微生物进行分类。一、利用气相色谱法的可行性。
J Bacteriol. 1963 May;85(5):1039-44. doi: 10.1128/jb.85.5.1039-1044.1963.
2
Mass spectrometry in lipid research.脂质研究中的质谱分析。
J Lipid Res. 1960 Oct;1:361-90.
3
Occurrence of 3-hydroxytridecanoic and 3-hydroxypentadecanoic acids in the lipopolysaccharides of Veillonella.
Biochim Biophys Acta. 1971 Mar 16;231(2):274-6. doi: 10.1016/0005-2760(71)90138-x.
4
Production of an artifact during methanolysis of lipids by boron trifluoride-methanol.在脂质的三氟化硼 - 甲醇甲醇解过程中产生的一种假象产物。
J Lipid Res. 1970 May;11(3):276-7.
5
On methylation of unsaturated acids using boron trihalide-methanol reagents.关于使用三卤化硼-甲醇试剂对不饱和酸进行甲基化反应
J Lipid Res. 1971 Nov;12(6):773-6.
6
7
Characterization of five clostridial pathogens by gas-liquid chromatography.
Am J Vet Res. 1970 Oct;31(10):1863-6.
8
9
Nature and linkages of the fatty acids present in lipopolysaccharides from Vibrio metchnikovii and Vibrio parahemolyticus.米氏弧菌和副溶血性弧菌脂多糖中脂肪酸的性质及联系
Eur J Biochem. 1973 Aug 1;37(1):116-20. doi: 10.1111/j.1432-1033.1973.tb02965.x.
10
Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia.嗜麦芽窄食单胞菌中支链羟基脂肪酸的存在情况。
J Bacteriol. 1973 Jun;114(3):1018-24. doi: 10.1128/jb.114.3.1018-1024.1973.
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