细菌脂肪酸分析快速方法的比较
Comparison of rapid methods for analysis of bacterial fatty acids.
作者信息
Moss C W, Lambert M A, Merwin W H
出版信息
Appl Microbiol. 1974 Jul;28(1):80-5. doi: 10.1128/am.28.1.80-85.1974.
Abstract
When rapid gas-liquid chromatography methods for determination of bacterial fatty acids were compared, results showed that saponification was required for total fatty acid analysis. Transesterification with boron-trihalide reagents (BF(3)-CH(3)OH, BCl(3)-CH(3)OH) caused extensive degradation of cyclopropane acids and was less effective than saponification in releasing cellular hydroxy fatty acids. Digestion of cells with tetramethylammonium hydroxide was unsatisfactory because of extraneous gas-liquid chromatography peaks and because of lower recovery of branched-chain and hydroxy fatty acids. A simple, rapid saponification procedure which can be used for total cellular fatty acid analysis of freshly grown cells is described.
摘要
在比较用于测定细菌脂肪酸的快速气液色谱法时，结果表明总脂肪酸分析需要皂化。用三卤化硼试剂（BF(3)-CH(3)OH、BCl(3)-CH(3)OH）进行酯交换会导致环丙烷酸大量降解，并且在释放细胞羟基脂肪酸方面不如皂化有效。用氢氧化四甲铵消化细胞并不理想，因为会出现额外的气液色谱峰，并且支链脂肪酸和羟基脂肪酸的回收率较低。本文描述了一种简单、快速的皂化程序，可用于对新鲜培养细胞进行总细胞脂肪酸分析。
相似文献
1
Comparison of rapid methods for analysis of bacterial fatty acids.细菌脂肪酸分析快速方法的比较
Appl Microbiol. 1974 Jul;28(1):80-5. doi: 10.1128/am.28.1.80-85.1974.
2
Degradation of bacterial cyclopropane acids with boron trihalide reagents.用三卤化硼试剂降解细菌环丙烷酸
Microbios. 1977;18(71):51-8.
3
Cellular fatty acid composition of selected Pseudomonas species.所选假单胞菌属物种的细胞脂肪酸组成。
Appl Microbiol. 1972 Oct;24(4):596-8. doi: 10.1128/am.24.4.596-598.1972.
4
Gas chromatography of cyclopropane fatty acid methylesters prepared with methanolic boron trichloride and boron trifluoride.
J Chromatogr. 1972 Mar 22;66(1):138-40. doi: 10.1016/s0021-9673(01)82938-1.
5
Cellular fatty acids of Alcaligenes and Pseudomonas species isolated from clinical specimens.从临床标本中分离出的产碱菌属和假单胞菌属的细胞脂肪酸。
J Clin Microbiol. 1975 May;1(5):414-9. doi: 10.1128/jcm.1.5.414-419.1975.
6
On methylation of unsaturated acids using boron trihalide-methanol reagents.关于使用三卤化硼-甲醇试剂对不饱和酸进行甲基化反应
J Lipid Res. 1971 Nov;12(6):773-6.
7
Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids.通过细胞脂肪酸的气相色谱-质谱分析鉴定微生物。
J Chromatogr. 1975 Oct 29;112:594-604. doi: 10.1016/s0021-9673(00)99988-6.
8
Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens.从临床标本中获取的假单胞菌属的细胞脂肪酸和代谢产物。
J Clin Microbiol. 1976 Dec;4(6):492-502. doi: 10.1128/jcm.4.6.492-502.1976.
9
Beef, chicken and lamb fatty acid analysis--a simplified direct bimethylation procedure using freeze-dried material.牛羊肉和小羊肉脂肪酸分析——使用冻干材料简化的直接双甲酯化法。
Meat Sci. 2012 Dec;92(4):863-6. doi: 10.1016/j.meatsci.2012.06.013. Epub 2012 Jun 16.
10
Evaluation of a rapid method for preparation of fatty acid methyl esters for analysis by gas-liquid chromatography.用于气液色谱分析的脂肪酸甲酯快速制备方法的评估。
J Chromatogr. 1985 Aug 30;331(1):141-8. doi: 10.1016/0021-9673(85)80015-7.
引用本文的文献
1
Bioprospection of the trichomonacidal activity of lipid extracts derived from marine macroalgae Gigartina skottsbergii.从海洋大型海藻杉藻（Gigartina skottsbergii）的脂类提取物中进行杀滴虫活性的生物勘探。
PLoS One. 2023 May 8;18(5):e0285426. doi: 10.1371/journal.pone.0285426. eCollection 2023.
2
Assessment of Biochemical Composition and Antioxidant Properties of Algerian Date Palm ( L.) Seed Oil.阿尔及利亚海枣（L.）籽油的生化成分及抗氧化性能评估
Plants (Basel). 2022 Jan 29;11(3):381. doi: 10.3390/plants11030381.
3
Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells.南极大型藻类脂肪酸对人乳腺癌细胞生长的细胞毒性活性
Front Bioeng Biotechnol. 2018 Dec 3;6:185. doi: 10.3389/fbioe.2018.00185. eCollection 2018.
4
Evaluation of fatty acid profile, color characteristics, oxidative quality and stability of common Kilka () oil obtained by various extraction techniques.通过各种提取技术获得的常见基尔卡（）油的脂肪酸谱、颜色特征、氧化质量和稳定性评估。
J Food Sci Technol. 2017 May;54(6):1377-1383. doi: 10.1007/s13197-017-2549-0. Epub 2017 Mar 13.
5
Application of FAME (fatty acid methyl ester) analysis in the numerical taxonomic determination of bacterial guild structure.脂肪酸甲酯（FAME）分析在细菌菌门结构数值分类测定中的应用。
Microb Ecol. 1984 Sep;10(3):283-96. doi: 10.1007/BF02010941.
6
Growth and metabolic flexibility in groundwater bacteria.地下水细菌的生长和代谢灵活性。
Microb Ecol. 1989 Nov;18(3):235-48. doi: 10.1007/BF02075811.
7
Isotope-reinforced polyunsaturated fatty acids protect yeast cells from oxidative stress.同位素增强的多不饱和脂肪酸可保护酵母细胞免受氧化应激。
Free Radic Biol Med. 2011 Jan 1;50(1):130-8. doi: 10.1016/j.freeradbiomed.2010.10.690. Epub 2010 Oct 16.
8
Effects of heavy metal contamination upon soil microbes: lead-induced changes in general and denitrifying microbial communities as evidenced by molecular markers.重金属污染对土壤微生物的影响：铅诱导的普通微生物群落和反硝化微生物群落变化，分子标记证明
Int J Environ Res Public Health. 2008 Dec;5(5):450-6. doi: 10.3390/ijerph5050450.
9
Evaluation of a rapid method for the quantitative analysis of fatty acids in various matrices.一种用于各种基质中脂肪酸定量分析的快速方法的评估。
J Chromatogr A. 2008 Nov 28;1212(1-2):106-13. doi: 10.1016/j.chroma.2008.10.006. Epub 2008 Oct 8.
10
Combined Determination of Poly-beta-Hydroxyalkanoic and Cellular Fatty Acids in Starved Marine Bacteria and Sewage Sludge by Gas Chromatography with Flame Ionization or Mass Spectrometry Detection.采用火焰离子化或质谱检测气相色谱法联合测定饥饿海洋细菌和污水污泥中的聚β-羟基烷酸和细胞脂肪酸。
Appl Environ Microbiol. 1986 Oct;52(4):905-10. doi: 10.1128/aem.52.4.905-910.1986.
本文引用的文献
1
CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY.通过化学成分分析对微生物进行分类。一、利用气相色谱法的可行性。
J Bacteriol. 1963 May;85(5):1039-44. doi: 10.1128/jb.85.5.1039-1044.1963.
2
Mass spectrometry in lipid research.脂质研究中的质谱分析。
J Lipid Res. 1960 Oct;1:361-90.
3
Occurrence of 3-hydroxytridecanoic and 3-hydroxypentadecanoic acids in the lipopolysaccharides of Veillonella.
Biochim Biophys Acta. 1971 Mar 16;231(2):274-6. doi: 10.1016/0005-2760(71)90138-x.
4
Production of an artifact during methanolysis of lipids by boron trifluoride-methanol.在脂质的三氟化硼 - 甲醇甲醇解过程中产生的一种假象产物。
J Lipid Res. 1970 May;11(3):276-7.
5
On methylation of unsaturated acids using boron trihalide-methanol reagents.关于使用三卤化硼-甲醇试剂对不饱和酸进行甲基化反应
J Lipid Res. 1971 Nov;12(6):773-6.
6
7
Characterization of five clostridial pathogens by gas-liquid chromatography.通过气液色谱法对五种梭状芽孢杆菌病原体进行鉴定。
Am J Vet Res. 1970 Oct;31(10):1863-6.
8
9
Nature and linkages of the fatty acids present in lipopolysaccharides from Vibrio metchnikovii and Vibrio parahemolyticus.米氏弧菌和副溶血性弧菌脂多糖中脂肪酸的性质及联系
Eur J Biochem. 1973 Aug 1;37(1):116-20. doi: 10.1111/j.1432-1033.1973.tb02965.x.
10
Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia.嗜麦芽窄食单胞菌中支链羟基脂肪酸的存在情况。
J Bacteriol. 1973 Jun;114(3):1018-24. doi: 10.1128/jb.114.3.1018-1024.1973.
Zotero 插件