Dees S B, Moss C W
J Clin Microbiol. 1975 May;1(5):414-9. doi: 10.1128/jcm.1.5.414-419.1975.
The cellular fatty acid composition of 25 clinical isolates of Alcaligenes and Pseudomonas was determined by gas-liquid chromatography (GLC). The GLC fatty acid profiles of three species of Pseudomonas were markedly different from those of Alcaligenes. The most significant differences were the presence and relative amounts of hydroxy, branched-chain, and cyclopropane fatty acids. One of the major fatty acids in A. faecalis was a 17-carbon cyclopropane (17 delta) acid, whereas a 15-carbon branched-chain acid (13-methyl tetradecanoate) characterized isolates of P. putrefaciens. The determination of these fatty acids by GLC provides a rapid and specific means of distinguishing clinical isolates of Pseudomonas and Alcaligenes.
采用气液色谱法(GLC)测定了25株产碱杆菌属和假单胞菌属临床分离株的细胞脂肪酸组成。三种假单胞菌的GLC脂肪酸谱与产碱杆菌属明显不同。最显著的差异在于羟基、支链和环丙烷脂肪酸的存在及相对含量。粪产碱杆菌的主要脂肪酸之一是17碳环丙烷(17δ)酸,而腐败假单胞菌分离株的特征性脂肪酸是15碳支链酸(13-甲基十四烷酸)。通过GLC测定这些脂肪酸为区分假单胞菌属和产碱杆菌属的临床分离株提供了一种快速且特异的方法。